CAJ | 학술논문

目的：研究诱骗受体3（DcR3）在正常大鼠、糖尿病（DM）大鼠心肌组织的表达，DcR3重组蛋白对心肌组织凋亡相关分子表达以及心肌细胞凋亡的影响，探讨DcR3对DM大鼠心肌细胞凋亡的作用。方法：一次性腹腔注射链脲佐菌素建立大鼠DM模型，尾静脉注射不同剂量的DcR3重组蛋白[1．2 mg／（鼠? d）、0．8 mg／（鼠? d）、0．4 mg／（鼠? d）]40 d。 RT－PCR检测心肌组织DcR3 mRNA、Fas mRNA、FasL mRNA的表达。 Wester blot分析凋亡相关蛋白Bcl－2、Caspase－8的表达。双抗夹心ELISA检测血液中IL－1β、TNF－α及LFN－γ水平的变化，HE染色观察心肌细胞凋亡的百分率。结果：DcR3治疗组大鼠与DM组比较心肌组织DcR3 mRNA高表达，Fas mRNA、FasL mRNA表达下调。 Caspase－8蛋白水平下调，Bcl－2蛋白水平上调，以中剂量组的作用最明显。各DcR3治疗组血清IL－1β、TNF－α和IFN－γ水平均有不同程度的降低（P＜0．05，P＜0．01）。心肌细胞凋亡的百分率下降（P＜0．05）。结论：DcR3重组蛋白有抑制DM大鼠心肌细胞凋亡的作用，其机制与竞争Fas，阻断FasL诱导细胞凋亡，心肌细胞表达DcR3，凋亡相关因子Caspase－8下调、Bcl－2上调及细胞因子水平的降低有关。
목적：연구유편수체3（DcR3）재정상대서、당뇨병（DM）대서심기조직적표체，DcR3중조단백대심기조직조망상관분자표체이급심기세포조망적영향，탐토DcR3대DM대서심기세포조망적작용。방법：일차성복강주사련뇨좌균소건립대서DM모형，미정맥주사불동제량적DcR3중조단백[1．2 mg／（서? d）、0．8 mg／（서? d）、0．4 mg／（서? d）]40 d。 RT－PCR검측심기조직DcR3 mRNA、Fas mRNA、FasL mRNA적표체。 Wester blot분석조망상관단백Bcl－2、Caspase－8적표체。쌍항협심ELISA검측혈액중IL－1β、TNF－α급LFN－γ수평적변화，HE염색관찰심기세포조망적백분솔。결과：DcR3치료조대서여DM조비교심기조직DcR3 mRNA고표체，Fas mRNA、FasL mRNA표체하조。 Caspase－8단백수평하조，Bcl－2단백수평상조，이중제량조적작용최명현。각DcR3치료조혈청IL－1β、TNF－α화IFN－γ수평균유불동정도적강저（P＜0．05，P＜0．01）。심기세포조망적백분솔하강（P＜0．05）。결론：DcR3중조단백유억제DM대서심기세포조망적작용，기궤제여경쟁Fas，조단FasL유도세포조망，심기세포표체DcR3，조망상관인자Caspase－8하조、Bcl－2상조급세포인자수평적강저유관。
Objective:To study the expression of DcR3 of myocardial tissue in diabetic mouse and normal rats and the impact of DcR3 recombinant protein to the expression of related molecules and myocardial cell apoptosis to discuss the action of DcR3 to myocardial cell apoptosis in Diabetic rats.Methods:Intraperitoneally injected streptozotocin one time to establish the model of Diabetic rats.Injected different doses of DcR3 recombinant protein to tail vein[1.2 mg/(rat? d),0.8 mg/(rat? d),0.4 mg/(rat? d)] 40 d. The expression of DcR3 mRNA, Fas mRNA and FasL mRNA of myocardial tissue was detected with RT-PCR;the expression of apoptosis related molecules Bcl-2 and Caspase-8 was analyzed with Western blot;the IL-1β, TNF-αand LFN-γof the blood was detected with double antibody sandwich ELISA;the percentage of myocardial cell apoptosis was observed with HE dyeing.Results:To compare the DcR3 treatment group with diabetic group,the expression DcR3 of myocardial tissue was high,the expression of Fas mRNA and FasL mRNA was descended.The Caspase-8 protein was ascended and the Bcl-2 protein was descended.The middle dose group was the most obvious.the IL-1β,TNF-αand IFN-γin the blood was descended differently in each DcR3 treatment group(P<0.05,P<0.01).The percentage of myocardial cell apoptosis was declined(P<0.05).Conclusion:DcR3 recombinant protein have the action of inhibiting the rats′myocardial cell apoptosis,the mechanism is related to competing with Fas,blocking-up FasL of inducing apoptosis, expressing DcR3 of myocardial cell,the descending of apoptosis related factors Caspase-8,the ascending of Bcl-2 and the reduction of cytokine levels.