CAJ | 학술논문

目的：观察EAPS疾病进展过程中小鼠外周血Th17细胞与CD4＋CD25＋调节性T（ Treg）细胞的比率变化。方法：以重组人β2糖蛋白1（ rhβ2 GP1）主动免疫BALB／c小鼠建立EAPS模型，免疫12周后检测外周血浆抗β2糖蛋白1抗体（anti－β2GP1）、抗心磷脂抗体（aCA）、IL－17、IL－2、IL－6、TGF－β、活化部分凝血活酶时间（APTT）和血小板计数（PLT）及流产率。流式细胞术（ FCM）检测小鼠PBMC中CD4＋CD25＋Treg及Th17细胞的比率。结果：与对照组相比，模型小鼠anti－β2 GP1、aCA、IL－17、IL－2、IL－6水平明显升高，APTT延长，TGF－β降低，PLT升高，流产率提高，差异有统计学意义（ P＜0．05）。 PBMC中CD4＋CD25＋Treg细胞频率8周前与对照组相比差异无统计学意义（P＞0．05），12周后逐渐减少，明显低于对照组（P＜0．05）；Th17细胞频率逐渐升高，明显高于对照组（P＜0．05）；CD4＋CD25＋Treg／Th17比值明显低于对照组（P＜0．05）。结论：EAPS小鼠外周血Th17／Treg细胞比率失衡可能在EAPS的发生发展中起重要作用。
목적：관찰EAPS질병진전과정중소서외주혈Th17세포여CD4＋CD25＋조절성T（ Treg）세포적비솔변화。방법：이중조인β2당단백1（ rhβ2 GP1）주동면역BALB／c소서건립EAPS모형，면역12주후검측외주혈장항β2당단백1항체（anti－β2GP1）、항심린지항체（aCA）、IL－17、IL－2、IL－6、TGF－β、활화부분응혈활매시간（APTT）화혈소판계수（PLT）급유산솔。류식세포술（ FCM）검측소서PBMC중CD4＋CD25＋Treg급Th17세포적비솔。결과：여대조조상비，모형소서anti－β2 GP1、aCA、IL－17、IL－2、IL－6수평명현승고，APTT연장，TGF－β강저，PLT승고，유산솔제고，차이유통계학의의（ P＜0．05）。 PBMC중CD4＋CD25＋Treg세포빈솔8주전여대조조상비차이무통계학의의（P＞0．05），12주후축점감소，명현저우대조조（P＜0．05）；Th17세포빈솔축점승고，명현고우대조조（P＜0．05）；CD4＋CD25＋Treg／Th17비치명현저우대조조（P＜0．05）。결론：EAPS소서외주혈Th17／Treg세포비솔실형가능재EAPS적발생발전중기중요작용。
Objective:To observe the change of CD4+CD25+regulatory T cells and Th17 cells in mice with experimental anti-phospholipid antibody syndrome ( EAPS ) .Methods: EAPS model was established by immunizing BALB/c mice with recombinant humanβ2 glycoprotein 1 (rhβ2GP1).The levels of serum anti-β2 glycoprotein 1 (anti-β2GP1),anti-cardiolipin antibody (aCA),IL-17,IL-2,IL-6 and TGF-βwere tested by ELISA.The rate of abortion,activated partial thromboplastin time (APTT) and platelet count were also detected.Flow cytometry was applied to detect the percentages of the CD4+CD25+regulatory T cells and Th17 cells in peripheral blood mononuclear cells.Results:Compared with the control group,the levels of anti-β2 GP1,aCA,IL-17,IL-2 and IL-6 were significantly increased,the rate of abortion was increased,APTT time was prolonged and the levels of TGF-βand platelet count were de-creased in model mice (P<0.05).No significant difference was detected of percentage of Treg cells in PBMC at the eighth weeks in model group (P>0.05),but percentage of Treg cells was lower than that in control group after 12 weeks (P<0.05);the percentage of Th17 cells in model group was higher than that in control group (P<0.05).In addition,the ratio of Treg/Th17 cells was lower in model mice than that in control group.Conclusion: The imbalance of CD4+CD25 Treg/Th17 cells may participate in the pathogenesis of EAPS.