CAJ | 학술논문

目的：观察病毒性心肌炎（VMC）小鼠急性期不同时点白细胞介素21（IL－21）的表达及其与抗腺嘌呤核苷酸转位子（ adenine nucleotide translocator，ANT）抗体的相关性，探究其在VMC发病中的作用及意义。方法：BALB／c小鼠腹腔注射柯萨奇病毒B3（ CVB3）建立VMC模型，腹腔注射等量PBS的BALB／c小鼠作为对照组，在注射的第0、1、2、3、4和6周，应用荧光定量逆转录－聚合酶链反应（ qRT－PCR）检测小鼠心肌组织中IL－21mRNA的表达、酶联免疫双夹心抗体法（ ELISA）检测血清IL－21蛋白及抗ANT抗体的表达。结果：与对照组比较，VMC小鼠心肌组织中IL－21mRNA及血清中IL－21自1周开始高表达，2周时达峰值，并维持高表达至6周，各时点表达量均高于相应时点的对照组（ P＜0．05）。血清中抗ANT抗体在第1周时开始升高，2周时达峰值，维持高水平表达至第6周，除0周时点外，VMC小鼠与对照组小鼠比较差异均有统计学意义（ P＜0．05），Pearson相关分析显示，IL－21与抗ANT抗体存在相关性（r＝0．88，P＜0．01）。结论：IL－21在VMC小鼠心肌组织及外周血中高表达，并与血清抗ANT抗体呈正相关，提示IL－21参与VMC的发病过程。
목적：관찰병독성심기염（VMC）소서급성기불동시점백세포개소21（IL－21）적표체급기여항선표령핵감산전위자（ adenine nucleotide translocator，ANT）항체적상관성，탐구기재VMC발병중적작용급의의。방법：BALB／c소서복강주사가살기병독B3（ CVB3）건립VMC모형，복강주사등량PBS적BALB／c소서작위대조조，재주사적제0、1、2、3、4화6주，응용형광정량역전록－취합매련반응（ qRT－PCR）검측소서심기조직중IL－21mRNA적표체、매련면역쌍협심항체법（ ELISA）검측혈청IL－21단백급항ANT항체적표체。결과：여대조조비교，VMC소서심기조직중IL－21mRNA급혈청중IL－21자1주개시고표체，2주시체봉치，병유지고표체지6주，각시점표체량균고우상응시점적대조조（ P＜0．05）。혈청중항ANT항체재제1주시개시승고，2주시체봉치，유지고수평표체지제6주，제0주시점외，VMC소서여대조조소서비교차이균유통계학의의（ P＜0．05），Pearson상관분석현시，IL－21여항ANT항체존재상관성（r＝0．88，P＜0．01）。결론：IL－21재VMC소서심기조직급외주혈중고표체，병여혈청항ANT항체정정상관，제시IL－21삼여VMC적발병과정。
Objective:To explore the expression of IL-21 and the relationship with adenine nucleotide translocator antibody, ( ANT antibody ) in different courses of viral myocarditis ( VMC ) mice, and to explore the role and significance of IL-21 in VMC.Methods:To induced VMC mice model, BALB/c male mice were peritoneally injected ( IP ) with Coxsackievirus B3, mice peritoneally injected with PBS were taken as the controls.On 0,1,2,3,4 and 6 w after IP,the level of IL-21 mRNA in mice myocardic tissue was determined by real-time RT-PCR.The expression of IL-21 protein and ANT antibody in serum was evaluated by EILSA.Results:Comparing with the controls,the expression of IL-21 mRNA and protein increased from 1st week after IP,reaching the peak on the 2nd week,and maintaining a higher trend until 6th week.All the results at each time except that of 0 week were higher than those of controls(P<0.05).The levels of ANT antibody were also increased from 1st to 6th week,reaching the peak on the 2nd week all the results at each time except that of 0 week were higher than those of controls(P<0.05).Pearson analysis showed that there was rela-tionship between IL-21 and ANT antibody( r=0.88,P<0.01).Conclusion:The datas show that local significantly increased levels of IL-21 in myocardium and serum, and have positive relationship with the level of adenine nucleotide translocator antibody, which indicates that IL-21 may play a role in the pathogenesis of mice virus myocarditis.