CAJ | 학술논문

目的：建立戒酒灵口服液中葫芦素B和葫芦素E的TLC鉴别和HPLC含量测定的方法，为建立戒酒灵口服液质量标准提供参考。方法采用TLC法对戒酒灵口服液中葫芦素B和葫芦素E进行定性鉴别，并采用HPLC法对二者进行含量测定。色谱条件：COSMOSIL C18色谱柱（250 mm ×4.6 mm，5μm）；乙腈（A）和0.2％醋酸-水溶液（B）梯度洗脱（0.0～15.0 min：30％～40％ A；15.0～40.0 min：40％～45％A）；检测波长为234 nm；柱温为室温；流速为1 mL／min；进样量为10μL。结果在TLC图中，戒酒灵口服液的供试品溶液分别与葫芦素B、E的对照品溶液在相同位置上显现暗斑。含量测定实验中，葫芦素B和葫芦素E质量浓度分别在0.01574～0.3148 mg／mL和0.003744～0.07488 mg／mL范围内与峰面积呈良好的线性关系，平均回收率分别为97.7％（RSD＝1.29％，n＝6）和98.4％（RSD＝1.68％，n＝6）。戒酒灵口服液中葫芦素B和葫芦素E的平均含量分别为0.36 mg／mL（ RSD＝0.85％，n＝6）和0.033 mg／mL（ RSD＝0.78％，n＝6）。结论此鉴别和含量测定方法简便、快捷、准确、可靠，能有效地对戒酒灵口服液的质量进行控制。
목적：건립계주령구복액중호호소B화호호소E적TLC감별화HPLC함량측정적방법，위건립계주령구복액질량표준제공삼고。방법채용TLC법대계주령구복액중호호소B화호호소E진행정성감별，병채용HPLC법대이자진행함량측정。색보조건：COSMOSIL C18색보주（250 mm ×4.6 mm，5μm）；을정（A）화0.2％작산-수용액（B）제도세탈（0.0～15.0 min：30％～40％ A；15.0～40.0 min：40％～45％A）；검측파장위234 nm；주온위실온；류속위1 mL／min；진양량위10μL。결과재TLC도중，계주령구복액적공시품용액분별여호호소B、E적대조품용액재상동위치상현현암반。함량측정실험중，호호소B화호호소E질량농도분별재0.01574～0.3148 mg／mL화0.003744～0.07488 mg／mL범위내여봉면적정량호적선성관계，평균회수솔분별위97.7％（RSD＝1.29％，n＝6）화98.4％（RSD＝1.68％，n＝6）。계주령구복액중호호소B화호호소E적평균함량분별위0.36 mg／mL（ RSD＝0.85％，n＝6）화0.033 mg／mL（ RSD＝0.78％，n＝6）。결론차감별화함량측정방법간편、쾌첩、준학、가고，능유효지대계주령구복액적질량진행공제。
Objective To establish an effective TLC and HPLC method for qualitative and quantitative analysis of cucurbitacin B and cucurbitacin E in JIE JIU LING oral liquid , which can be used to control the quality of JIE JIU LING oral liquid .Methods Cucurbitacin B and cucurbitacin E in JIE JIU LING oral liquid were identified and determined by TLC and HPLC . The conditions of HPLC included the column of COSMOSIL C 18 column (250 mm ×4.6 mm, 5 μm), the mobile phase of acetonitrile-0.2%acetic acid solution (0.0~15.0 min:30%~40%A;15.0~40.0 min:40%~45% A) , a flow rate of 1.0 mL/min, the detection wave-length of 234 nm.Results In contrast to the control solution of cucurbitacin B and cucurbita-cin E, the sample solution of JIE JIU LING oral liquid appeared dark spots in the same position of TLC, respectively .The linear ranges of cucurbitacin B and cucurbitacin E were 0.015 74~0.314 8 mg/mL and 0.003 744~0.074 88 mg/mL, respectively .The average recovery of cu-curbitacin B and cucurbitacin E were 97.7% ( RSD=1.29%, n=6 ) and 98.4% ( RSD=1.68%, n=6), respectively.The average content of cucurbitacin B and cucurbitacin E in JIE JIU LING oral liquid were 0.36 mg/mL ( RSD=0.85%, n=6 ) and 0.033mg /mL ( RSD=0.78%, n=6), respectively.Conclusion The methods of TLC and HPLC are reliable, sta-ble and repeatable , which can be applied as the quantity control method of cucurbitacin B and cucurbitacin E in JIE JIU LING oral liquid .