CAJ | 학술논문

克隆和分析橘青霉 Penicillium citrinum的全局性调控因子Pci‐veA 基因．设计简并引物，用PCR扩增获得Pci‐veA 基因的核心片段，利用RACE和TAIL‐PCR技术对核心片段的3’和5’末端进行延伸，将获得的3个片段进行拼接并设计引物，克隆得到完整的 Pci‐veA 基因．结果表明：Pci‐veA 基因全长1774 bp ，包含一个70 bp的内含子，cDNA序列为1704 bp ，编码567个氨基酸；Pci‐veA 与多种已报道的真菌veA 基因具有较高的同源性，且含有 veA基因特定的双组份核蛋白定位信号区NLSI和Pat7定位信号区域．这些结果为进一步研究 P. citrinum中美伐他汀生物合成的全局调控机理奠定了基础．
극륭화분석귤청매 Penicillium citrinum적전국성조공인자Pci‐veA 기인．설계간병인물，용PCR확증획득Pci‐veA 기인적핵심편단，이용RACE화TAIL‐PCR기술대핵심편단적3’화5’말단진행연신，장획득적3개편단진행병접병설계인물，극륭득도완정적 Pci‐veA 기인．결과표명：Pci‐veA 기인전장1774 bp ，포함일개70 bp적내함자，cDNA서렬위1704 bp ，편마567개안기산；Pci‐veA 여다충이보도적진균veA 기인구유교고적동원성，차함유 veA기인특정적쌍조빈핵단백정위신호구NLSI화Pat7정위신호구역．저사결과위진일보연구 P. citrinum중미벌타정생물합성적전국조공궤리전정료기출．
To clone the global regulator Pci‐veA of Penicillium citrinum .Based on the conserved sequence region of gene veA reported from fungus ,the core fragment of gene Pci‐veA was cloned from P .citrinum mycelial samples by using degenerate primers amplify method .And ,the 3'cDNA and 5'cDNA ends of Pci‐veA were amplified using RACE and TAIL‐PCR methods .The full‐length sequence of Pci‐veA gene is 1 774 bp ,w hich contains an intron with length of 70 bp and an open reading frame (ORF) encoding a pro‐tein with 567 amino acids .Pci‐veA shows highly homologue with several reported veA genes ,and contains specific motifs of bipartite nuclear localization signal motif NLSI and pat 7 motif .These results suggest that Pci‐veA will be useful for understanding of molecular regulation mechanism of mevastatin biosynthesis in P .citrinum .