CAJ | 학술논문

目的：探讨非压迫性椎间盘髓核突出中自身免疫反应对神经根损伤的作用机制及临床意义。方法：雌性SD大鼠48只，随机分成模型组、对照组，每组24只。造模后10 d、20 d、40 d，每组随机抽取8只，测定左后肢痛阈；检测动脉血中CD4＋、CD8＋T细胞比例及血清中TNF－α表达；HE染色观察神经根形态学变化。结果：模型组在10 d、20 d时，左后肢痛阈、CD4＋、CD8＋T细胞比例及TNF－α表达与对照组比较，差异均有统计学意义，在40 d时两组比较，差异均无统计学差异。模型组10 d时腰神经根横断面髓鞘部分崩解，呈空泡变；20 d时最严重，40 d时基本恢复正常；对照组未见异常。结论：在非压迫性椎间盘髓核突出早期，T细胞介导的自身免疫及TNF－α对神经根损伤发挥重要作用。
목적：탐토비압박성추간반수핵돌출중자신면역반응대신경근손상적작용궤제급림상의의。방법：자성SD대서48지，수궤분성모형조、대조조，매조24지。조모후10 d、20 d、40 d，매조수궤추취8지，측정좌후지통역；검측동맥혈중CD4＋、CD8＋T세포비례급혈청중TNF－α표체；HE염색관찰신경근형태학변화。결과：모형조재10 d、20 d시，좌후지통역、CD4＋、CD8＋T세포비례급TNF－α표체여대조조비교，차이균유통계학의의，재40 d시량조비교，차이균무통계학차이。모형조10 d시요신경근횡단면수초부분붕해，정공포변；20 d시최엄중，40 d시기본회복정상；대조조미견이상。결론：재비압박성추간반수핵돌출조기，T세포개도적자신면역급TNF－α대신경근손상발휘중요작용。
Objective:To discuss the autoimmune response on the mechanism and clinical significance of nerve root injury induced by the non-compressive nucleus pulposus protrusion.Methods:Forty-eight female SD rats were randomly divided.Twenty-four female SD rats were recruited as model group,and the others as control.After ten days,twenty days and forty days,the pain threshold of left hind leg and the levels of TNF-α, CD4+, CD8+T cells were measured, the nerve root changes in morphology were observed by HE.Results:Compared with the control group,model group in ten days,twenty days,which left hind leg pain threshold,the proportion of CD4,CD8+T cell and the expression of TNF-αare significantly different.After forty days,these two groups have no statistical differ-ence.After ten days,lumbar nerve root cross-sectional myelin of model group was partial disintegrated.The worst damage happened in twenty days,and almost recovered to normal in forty days.Conclusion:T cell-mediated autoimmunity and TNF-αplay an important role to the nerve root injury in the early time of the non-compressive nucleus pulposus protrusion.