哈尔滨医科大学学报
哈爾濱醫科大學學報
합이빈의과대학학보
JOURNAL OF HARBIN MEDICAL UNIVERSITY
2015年
2期
127-130
,共4页
董伟%田淑君%魏志%朱述英%陈丙春%王海涛
董偉%田淑君%魏誌%硃述英%陳丙春%王海濤
동위%전숙군%위지%주술영%진병춘%왕해도
大肠癌%凋亡%PP2 A%Noxa
大腸癌%凋亡%PP2 A%Noxa
대장암%조망%PP2 A%Noxa
Kye words:colorectal cancer%apoptosis%PP2A%Noxa
目的:检测PP2 A及Noxa蛋白在大肠癌癌组织和癌旁组织中的表达,探讨PP2 A、Noxa在大肠癌发生、发展中的作用以及二者的相关性。方法采用免疫组织化学法检测40例大肠癌患者的癌组织(大肠癌组)及癌旁组织(癌旁组) PP2A、Noxa蛋白表达情况,采用TUNEL法检测细胞凋亡情况。结果大肠癌组PP2A与Noxa蛋白阳性表达率(90%、82.5%)明显低于癌旁组(95%、88.1%),P<0.05;大肠癌组PP2A、Noxa蛋白阳性表达在大肠癌组织分化程度、淋巴结转移差异有统计学意义( P<0.05);40例大肠癌组织中PP2A蛋白与Noxa蛋白表达之间呈正相关(r=0.229,P<0.05)。大肠癌癌细胞凋亡指数在癌细胞分化程度、淋巴结转移及临床分期上差异有统计学意义( P<0.05);大肠癌组PP2A、Noxa表达阴性者癌细胞凋亡指数均明显低于表达阳性者( P<0.05)。结论 PP2 A、Noxa蛋白可能相互协同,在结直肠癌的发生、发展过程中发挥抑制作用。
目的:檢測PP2 A及Noxa蛋白在大腸癌癌組織和癌徬組織中的錶達,探討PP2 A、Noxa在大腸癌髮生、髮展中的作用以及二者的相關性。方法採用免疫組織化學法檢測40例大腸癌患者的癌組織(大腸癌組)及癌徬組織(癌徬組) PP2A、Noxa蛋白錶達情況,採用TUNEL法檢測細胞凋亡情況。結果大腸癌組PP2A與Noxa蛋白暘性錶達率(90%、82.5%)明顯低于癌徬組(95%、88.1%),P<0.05;大腸癌組PP2A、Noxa蛋白暘性錶達在大腸癌組織分化程度、淋巴結轉移差異有統計學意義( P<0.05);40例大腸癌組織中PP2A蛋白與Noxa蛋白錶達之間呈正相關(r=0.229,P<0.05)。大腸癌癌細胞凋亡指數在癌細胞分化程度、淋巴結轉移及臨床分期上差異有統計學意義( P<0.05);大腸癌組PP2A、Noxa錶達陰性者癌細胞凋亡指數均明顯低于錶達暘性者( P<0.05)。結論 PP2 A、Noxa蛋白可能相互協同,在結直腸癌的髮生、髮展過程中髮揮抑製作用。
목적:검측PP2 A급Noxa단백재대장암암조직화암방조직중적표체,탐토PP2 A、Noxa재대장암발생、발전중적작용이급이자적상관성。방법채용면역조직화학법검측40례대장암환자적암조직(대장암조)급암방조직(암방조) PP2A、Noxa단백표체정황,채용TUNEL법검측세포조망정황。결과대장암조PP2A여Noxa단백양성표체솔(90%、82.5%)명현저우암방조(95%、88.1%),P<0.05;대장암조PP2A、Noxa단백양성표체재대장암조직분화정도、림파결전이차이유통계학의의( P<0.05);40례대장암조직중PP2A단백여Noxa단백표체지간정정상관(r=0.229,P<0.05)。대장암암세포조망지수재암세포분화정도、림파결전이급림상분기상차이유통계학의의( P<0.05);대장암조PP2A、Noxa표체음성자암세포조망지수균명현저우표체양성자( P<0.05)。결론 PP2 A、Noxa단백가능상호협동,재결직장암적발생、발전과정중발휘억제작용。
Objective To detect PP2 A and Noxa expression in colorectal carcinoma tissues and paracarcinoma tissues , and to explore the function and the correlation between PP 2A and Noxa in colorectal carcinoma .Methods PP2 A and Noxa protein in 40 colorectal carcinoma tissues and 40 paracancerous tissues were detected by immunohistochemical technique .The cell apop-tosis was detected by TUNEL .Results The positive expression of PP2A protein in colorectal cancer tissues was 90%, which was significantly lower than that of paracancerous tissues (95%)(P<0.05 );Noxa protein positive expression was 82.5% in colorectal cancer tissues vs 88.1%in paracarcinoma tissues (P<0.05).The rates of PP2A, Noxa protein expression was associated with the tumor differentiation , lymph node metastasis(P<0.05).Expression of PP2A protein and Noxa protein in 40 cases of colorectal carcinomas tissues showed a positivelinear correlation (r =0.229,P <0.05).The apoptosic index of colorectal cancer was statisti -cally significant in cell differentiation, lymph node metastasis and clinical stage.The apoptosicindex in PP2A, Noxa negative expression group was much lower than that in the positive group(P <0.05).Conclusion PP2A, Noxa proteins may play negative roles in the incidence anddevelopment of colorectal cancer, the correlation analysis between them is positive .