现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2015年
2期
68-69,73
,共3页
子痫前期%细胞凋亡%TFAR19
子癇前期%細胞凋亡%TFAR19
자간전기%세포조망%TFAR19
preeclampsia%apoptosis%TFAR19
目的：观察子痫前期患者血清 TFAR19活性。方法选取北京市怀柔区妇幼保健院住院初产行剖宫产术的子痫前期患者43例，其中重度子痫前期患者18例、轻度子痫前期患者25例，同时随机选取无妊娠并发症的初产剖宫产产妇40例设为对照组，于入院次日采集外周静脉血，采用酶联免疫吸附试验（enzyme-linked immunosorbent assay，ELISA）检测子痫前期患者血清中 TFAR19活性，即 TFAR19催化产生的吸光度 A。结果重度子痫前期、轻度子痫前期、正常对照组TFAR19活性分别为43.56±2.93，33.38±2.32和23.63±2.94活性单位，各组之间 TFAR19活性差异具有统计学意义（F ＝10.65，P ＜0.01）。与正常对照组相比，重度子痫前期组（q ＝6.384，P ＜0.001）、轻度子痫前期组（q ＝3.478，P ＝0.016）TFAR19活性差异均具有统计学意义。重度子痫前期组、轻度子痫前期组之间 TFAR19活性差异有统计学意义（q＝2.993，P ＝0.037）。各组之间年龄差异无统计学意义（F ＝0.091，P ＝0.913）。各组之间孕周、新生儿体重差异有统计学意义（F ＝4.37，P ＝0.016；F ＝35.06，P ＝0.00）。结论TFAR19的增加可促进机体细胞凋亡，参与子痫前期的发生，并且 TFAR19的含量可随病情的严重程度而增加。
目的：觀察子癇前期患者血清 TFAR19活性。方法選取北京市懷柔區婦幼保健院住院初產行剖宮產術的子癇前期患者43例，其中重度子癇前期患者18例、輕度子癇前期患者25例，同時隨機選取無妊娠併髮癥的初產剖宮產產婦40例設為對照組，于入院次日採集外週靜脈血，採用酶聯免疫吸附試驗（enzyme-linked immunosorbent assay，ELISA）檢測子癇前期患者血清中 TFAR19活性，即 TFAR19催化產生的吸光度 A。結果重度子癇前期、輕度子癇前期、正常對照組TFAR19活性分彆為43.56±2.93，33.38±2.32和23.63±2.94活性單位，各組之間 TFAR19活性差異具有統計學意義（F ＝10.65，P ＜0.01）。與正常對照組相比，重度子癇前期組（q ＝6.384，P ＜0.001）、輕度子癇前期組（q ＝3.478，P ＝0.016）TFAR19活性差異均具有統計學意義。重度子癇前期組、輕度子癇前期組之間 TFAR19活性差異有統計學意義（q＝2.993，P ＝0.037）。各組之間年齡差異無統計學意義（F ＝0.091，P ＝0.913）。各組之間孕週、新生兒體重差異有統計學意義（F ＝4.37，P ＝0.016；F ＝35.06，P ＝0.00）。結論TFAR19的增加可促進機體細胞凋亡，參與子癇前期的髮生，併且 TFAR19的含量可隨病情的嚴重程度而增加。
목적：관찰자간전기환자혈청 TFAR19활성。방법선취북경시부유구부유보건원주원초산행부궁산술적자간전기환자43례，기중중도자간전기환자18례、경도자간전기환자25례，동시수궤선취무임신병발증적초산부궁산산부40례설위대조조，우입원차일채집외주정맥혈，채용매련면역흡부시험（enzyme-linked immunosorbent assay，ELISA）검측자간전기환자혈청중 TFAR19활성，즉 TFAR19최화산생적흡광도 A。결과중도자간전기、경도자간전기、정상대조조TFAR19활성분별위43.56±2.93，33.38±2.32화23.63±2.94활성단위，각조지간 TFAR19활성차이구유통계학의의（F ＝10.65，P ＜0.01）。여정상대조조상비，중도자간전기조（q ＝6.384，P ＜0.001）、경도자간전기조（q ＝3.478，P ＝0.016）TFAR19활성차이균구유통계학의의。중도자간전기조、경도자간전기조지간 TFAR19활성차이유통계학의의（q＝2.993，P ＝0.037）。각조지간년령차이무통계학의의（F ＝0.091，P ＝0.913）。각조지간잉주、신생인체중차이유통계학의의（F ＝4.37，P ＝0.016；F ＝35.06，P ＝0.00）。결론TFAR19적증가가촉진궤체세포조망，삼여자간전기적발생，병차 TFAR19적함량가수병정적엄중정도이증가。
Objective To explore serum TFAR19 activity in preeclampsia patients.Methods 43 of cesarean delivery patients with preeclampsia in the Huairou Maternal and Child Health Hospital of Beijing were recruited and the peripheral venous blood was collected.Among them,severe preeclampsia patients were 18,mild preeclampsia were patients 25.In addition,40 unrelated healthy cesarean puerpera from a routine health survey were enrolled as controls.Enzyme-linked immunosorbent assay(ELISA)was used to detect serum TFAR19 activity in preeclampsia patients.That was absorbancy-A catalytic genera-ted by TFAR19.Results The activity of TFAR19 severe preeclampsia patients,mild preeclampsia patients,and healthy con-trols were 43.56±2.93,33.38±2.32 and 23.63±2.94,respectively.The TFAR19 activity in each group was statistically significant difference (F = 10.65,P <0.01).Compared with healthy controls,the TFAR19 activity in severe preeclampsia group (q=6.384,P <0.001),mild preeclampsia group (q = 3.478,P =0.016)was statistically significant difference.The TFAR19 activity in preeclampsia patients subgroups (severe preeclampsia group,mild preeclampsia group)was statistically significant difference (q = 2.993,P = 0.037).Age between each groups was no-statistically significant difference (F =0.091,P =0.913).Gestation age and neonatal weight betweeneach groups was statistically significant difference (F=4.37, P =0.016;F=35.06,P <0.001).Conclusion The increase of TFAR19 could promote apoptosis,participating the occur-rence of preeclampsia.The content of TFAR19 could be increased with the severity of the preeclampsia.
