CAJ | 학술논문

目的：通过检测银屑病患者血清中 IL-10与 IL-21表达水平，探讨其在银屑病病情进展程度及治疗效果中的意义。方法按照赵辨主编的临床皮肤病学诊断标准，选择2014年3月～9月期间皮肤科就诊的门诊和住院寻常型银屑病患者65例，其中进行期25例（A 组），稳定期20例（B 组），恢复期20例（C 组），并选择正常对照组25例（D 组）。ELISA 法检测各组血清中 IL-10与 IL-21表达水平，并对各组患者进行银屑病皮损面积和严重程度（PASI）评分。结果A 与 B 两组血清中 IL-21表达水平分别为127.59±16.09 pg／ml 和105.74±21.08 pg／ml，均明显高于 D 组85.46±14.25 pg／ml，差异有统计学意义（t 值分别为5.174，4.863，P 值均＜0.01），而两组 IL-10表达水平分别为10.64±3.23 pg／ml 和12.27±2.18 pg／ml，均低于 D 组20.29±2.51 pg／ml，差异有统计学意义（t 值分别为2.031，2.027，P 值均＜0.05）。C 组血清中IL-21表达水平94.03±8.90 pg／ml 高于 D 组，差异有统计学意义（t＝2.033，P ＜0.05）。A 组血清中 IL-21表达水平高于C 组，差异有统计学意义（t ＝2.352，P ＜0.05），IL-10表达水平低于 C 组19.69±1.54 pg／ml，差异有统计学意义（t ＝2.071，P ＜0.05）。银屑病患者血清中 IL-21表达水平与 PASI 评分值正相关（r＝0.508，P ＝0.027），而血清中 IL-10表达水平与 PASI 评分为负相关（r＝－0.413，P ＝0.039）。结论银屑病的病情进展与患者血清中 IL-21升高或 IL-10降低有关，检测细胞因子 IL-21和 IL-10水平有助于判断其病情严重程度及疗效评估。
목적：통과검측은설병환자혈청중 IL-10여 IL-21표체수평，탐토기재은설병병정진전정도급치료효과중적의의。방법안조조변주편적림상피부병학진단표준，선택2014년3월～9월기간피부과취진적문진화주원심상형은설병환자65례，기중진행기25례（A 조），은정기20례（B 조），회복기20례（C 조），병선택정상대조조25례（D 조）。ELISA 법검측각조혈청중 IL-10여 IL-21표체수평，병대각조환자진행은설병피손면적화엄중정도（PASI）평분。결과A 여 B 량조혈청중 IL-21표체수평분별위127.59±16.09 pg／ml 화105.74±21.08 pg／ml，균명현고우 D 조85.46±14.25 pg／ml，차이유통계학의의（t 치분별위5.174，4.863，P 치균＜0.01），이량조 IL-10표 체 수 평 분 별 위10.64±3.23 pg／ml 화12.27±2.18 pg／ml，균저우 D 조20.29±2.51 pg／ml，차이유통계학의의（t 치분별위2.031，2.027，P 치균＜0.05）。C 조혈청중IL-21표체수평94.03±8.90 pg／ml 고우 D 조，차이유통계학의의（t＝2.033，P ＜0.05）。A 조혈청중 IL-21표체수평고우C 조，차이유통계학의의（t ＝2.352，P ＜0.05），IL-10표체수평저우 C 조19.69±1.54 pg／ml，차 이 유 통 계 학 의의（t ＝2.071，P ＜0.05）。은설병환자혈청중 IL-21표체수평여 PASI 평분치정상관（r＝0.508，P ＝0.027），이혈청중 IL-10표체수평여 PASI 평분위부상관（r＝－0.413，P ＝0.039）。결론은설병적병정진전여환자혈청중 IL-21승고혹 IL-10강저유관，검측세포인자 IL-21화 IL-10수평유조우판단기병정엄중정도급료효평고。
Objective To detect the level of IL-10 and IL-21 in serum of patients with psoriasis and explore its significance of judge the extent of serious condition and treatment of psoriasis.Methods According to clinical dermatology edited by Zhao Bian diagnostic criteria,selected 65 cases of patients with psoriasis during the period from March to September in 2014 ad-mitted to Department of Dermatology of out-patient and hospitalization.25 cases were progressive stage (group A),20 pa-tients with stable stage (group B),20 patients were in recovery period (group C),and 25 cases of control group (group D). With ELISA method detect the expression level of IL-10 and IL-21 in serum of each group respectively.PASI score will take to all psoriasis patients meanwhile.Results The expressionlevels of IL-21 in serum of A (127.59 ± 16.09 pg/ml),B (105.74±21.08 pg/ml)two groups were significantly higher than that in D group (85.46±14.25 pg/ml),and the differ-ence were statistically significant (t=5.174,4.863,both P <0.01),while the expression levels of IL-10 in A (10.64±3.23 pg/ml)and B (12.27±2.18 pg/ml)were both lower than D group (20.29±2.51 pg/ml),and the difference were statisti-cally significant (t=2.031,2.027,both P <0.05).The expression of IL-21 (94.03±8.90 pg/ml)in C group was higher than that in D group,and the difference was statistically significant (t=2.033,P <0.05).The level of IL-21 in A group was higher than that in group C,and the difference was statistically significant (t=2.352,P <0.05),while the expression level of IL-10 was lower than that in C group (19.69±1.54 pg/ml),and the difference was statistically significant (t=2.071,P<0.05).The expression level of IL-21 in the serum of patients with psoriasis and PASI score was positively correlated (r=0.508,P =0.027),while the expression level of IL-10 and PASI score was negatively correlated (r=-0.413,P =0.039). Conclusion The progression of psoriasis is related to the increasion of IL-21 or the decreasion of IL-10.The detection of IL-21 and IL-10 was beneficial to judge the severity and efficacy evaluation of psoriasis.