CAJ | 학술논문

目的：研究同一患者体内甲状腺良性瘤与癌组织在 DNA 甲基化方面的差异，从而揭示 DNA 甲基化在肿瘤发生发展中的重要作用。方法：使用基因甲基化芯片技术对同一患者的并发甲状腺癌、甲状腺良性瘤组织样本的基因组甲基化水平进行检测，对筛选出的差异化显著的基因进行生物信息学分析得到功能基因的信号通路，并针对与甲状腺癌明显相关的信号通路进行分析讨论。结果：甲状腺的良性瘤与癌在DNA甲基化水平上存在显著差异；参与恶性肿瘤相关的信号通路包括T细胞受体的信号通路，Jak-STAT 信号通路与细胞因子－细胞因子受体相互作用信号通路等炎症相关信号通路。结论：研究得出炎症的相关生物信息学通路与甲状腺肿瘤的发生发展密切相关，为甲状腺恶性肿瘤的发生发展机制的阐明与潜在药物靶点的选择提供新的思路与方向。
목적：연구동일환자체내갑상선량성류여암조직재 DNA 갑기화방면적차이，종이게시 DNA 갑기화재종류발생발전중적중요작용。방법：사용기인갑기화심편기술대동일환자적병발갑상선암、갑상선량성류조직양본적기인조갑기화수평진행검측，대사선출적차이화현저적기인진행생물신식학분석득도공능기인적신호통로，병침대여갑상선암명현상관적신호통로진행분석토론。결과：갑상선적량성류여암재DNA갑기화수평상존재현저차이；삼여악성종류상관적신호통로포괄T세포수체적신호통로，Jak-STAT 신호통로여세포인자－세포인자수체상호작용신호통로등염증상관신호통로。결론：연구득출염증적상관생물신식학통로여갑상선종류적발생발전밀절상관，위갑상선악성종류적발생발전궤제적천명여잠재약물파점적선택제공신적사로여방향。
Objective To determine the possible differences in DNA methylation of benign and malignant thyroid tumors and its role in tumorgenesis and tumor progression. Methods Infinium human methylation 450 bead chip platform was utilized to screen the whole-genome of benign and malignant thyroid tumor tissues surgically removed from a patient for the determination of their DNA methylation levels. The genes with significant differences in methylation were analyzed for their functional pathways with bioinformatics. Results There were significant differences in DNA methylation between benign and malignant thyroid tumor. The inflammation-related pathways, such as T cell, Jak-STAT and cytokine-cytokine receptor signaling pathways involved in the biological process of tumorgenesis and tumor progression. Conclusions The differences in DNA methylation between benign and malignant reveal a close relationship between inflammation and tumorgenesis and tumor progression and provide a novel method for investigation of thyroid cancer development and target drug discovery.