草菇丝氨酸蛋白酶基因在贮藏期表达变化的研究
초고사안산단백매기인재저장기표체변화적연구
Expression of the Serine Protease Gene in Volvariella volvacea during Different Storage Time
  • 万方数据
    安徽农学通报 안휘농학통보
    AUHUI AGRICULTURAL SCIENCE BULLETIN
    2015年 8期 31-34 ,共4页

    逯连静%陈明杰%邢增涛%陈国荣

    록련정%진명걸%형증도%진국영

    草菇%实时荧光PCR%丝氨酸蛋白酶%贮藏期 草菇%實時熒光PCR%絲氨痠蛋白酶%貯藏期
    초고%실시형광PCR%사안산단백매%저장기
    Volvariellavolvacea%Real-time quantitative PCR%Perforated packaging film%Storage time
    采用不覆膜（control）与覆盖厚度为12μm、打6孔聚氯乙烯膜（PVC12-6）的方式分别对草菇进行保鲜贮藏，观察比较其蛋白酶活性，并通过实时荧光定量PCR技术研究草菇在贮藏期丝氨酸蛋白酶（SER）基因表达变化规律。结果表明：（1）control的草菇蛋白酶活性在储藏前期快速增加，在48h酶活性为3.81U/g·min，72h有所下降，但96h达到高峰，为5.80U/g·min；而PVC12-6的草菇蛋白酶活性在贮藏前期缓慢增加，在96h达到高峰，为2.76U/g·min，随后快速下降；在贮藏期间，control的草菇蛋白酶活性一直比PVC12-6高。（2）con?trol的丝氨酸蛋白酶基因表达量在48h达到最大，随后表达量下降较慢，且48h之后的表达量远高于24h的表达量；而PVC12-6的丝氨酸蛋白酶基因表达量在48h达到最高点后迅速下降，48h后的表达量远远低于24h的表达量。
    채용불복막（control）여복개후도위12μm、타6공취록을희막（PVC12-6）적방식분별대초고진행보선저장，관찰비교기단백매활성，병통과실시형광정량PCR기술연구초고재저장기사안산단백매（SER）기인표체변화규률。결과표명：（1）control적초고단백매활성재저장전기쾌속증가，재48h매활성위3.81U/g·min，72h유소하강，단96h체도고봉，위5.80U/g·min；이PVC12-6적초고단백매활성재저장전기완만증가，재96h체도고봉，위2.76U/g·min，수후쾌속하강；재저장기간，control적초고단백매활성일직비PVC12-6고。（2）con?trol적사안산단백매기인표체량재48h체도최대，수후표체량하강교만，차48h지후적표체량원고우24h적표체량；이PVC12-6적사안산단백매기인표체량재48h체도최고점후신속하강，48h후적표체량원원저우24h적표체량。
    The activity of protease of Volvariellavolvaceawhich were packaged by perforated packaging film were in?vestigated.The expression of the serine protease gene of the samples in different storage time were investigated by re?al-time quantitative PCR.The results showed that the fruit bodies with non-packaging retainedhighest activity of pro?tease which was 5.80U/g · min at 96h.Another sample reached thehighest activity of protease at 96h.The relative ex?pression of SER gene of all samples exhibited a rapid increase at 48h,then they were continuous decrease.But the rate of decreased expression in control was lower than another sample.This result was coincidence relation with the activity of protease.
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