CAJ | 학술논문

目的：研究急性淋巴细胞白血病（acute lymphoblastic leukemia， ALL）患者CDX2与β-catenin基因表达的相关性。方法：实时定量PCR方法检测43例初诊ALL患者CDX2及β-catenin mRNA的表达，以30例非恶性血液病患者作为对照。结果：ALL组CDX2 mRNA表达阳性率为93％，对照组无表达（P＜0．01）；β-catenin mRNA在两组均有表达，但ALL组显著高于对照组（P＜0．01）。 CDX2、β-catenin mRNA表达均与初诊时白细胞数、LDH水平呈正相关（P＜0．01）。ALL患者获得CR时，CDX2 mRNA、β-catenin mRNA表达显著下降，复发时两者均增高。相关性分析显示，ALL患者CDX2与β-catenin mRNA表达呈显著正相关（r＝0．835， P＝0．000）。结论：ALL患者存在CDX2基因过表达及Wnt／β-catenin信号通路激活，且CDX2与β-catenin表达呈显著正相关。
목적：연구급성림파세포백혈병（acute lymphoblastic leukemia， ALL）환자CDX2여β-catenin기인표체적상관성。방법：실시정량PCR방법검측43례초진ALL환자CDX2급β-catenin mRNA적표체，이30례비악성혈액병환자작위대조。결과：ALL조CDX2 mRNA표체양성솔위93％，대조조무표체（P＜0．01）；β-catenin mRNA재량조균유표체，단ALL조현저고우대조조（P＜0．01）。 CDX2、β-catenin mRNA표체균여초진시백세포수、LDH수평정정상관（P＜0．01）。ALL환자획득CR시，CDX2 mRNA、β-catenin mRNA표체현저하강，복발시량자균증고。상관성분석현시，ALL환자CDX2여β-catenin mRNA표체정현저정상관（r＝0．835， P＝0．000）。결론：ALL환자존재CDX2기인과표체급Wnt／β-catenin신호통로격활，차CDX2여β-catenin표체정현저정상관。
Objective To investigate the expressions of CDX2 and β-catenin in acute lymphoblastic leukemia (ALL) and their correlation. Methods Real-time PCR was used to determine the expressions of CDX2 and β-catenin mRNA in 43 de novo ALL and 30 non-malignant patients (used as control). Results The positivity rate of CDX2 mRNA expression in ALL group was 93%, but CDX2 mRNA expression couldn′t be detected in the control group (P<0.01). The mRNA expression of β-catenin could be detected in patients in both two groups, butβ-catenin mRNA expression in the ALL group was significantly higher than in the control group (P < 0.01). And mRNA expressions of CDX2 andβ-catenin were significantly correlated with WBC counts and LDH level (P<0.01). When the ALL patients acquired complete remission (CR), the mRNA expressions of CDX2 and β-catenin were significantly decreased compared with their newly diagnosed status , while disease-relapsed the mRNA expressions of CDX2 andβ-catenin were increased again. There was significantly positive correlation between CDX2 and β-catenin mRNA expressions (r = 0.835, P = 0.000). Conclusion Up-regulation of CDX2 and activation of Wnt/β-catenin pathway coexist in the ALL patients and the mRNA expressions of CDX2 and β-catenin are positively correlated.