CAJ | 학술논문

目的：研究体外吲哚菁绿（ICG）介导的光动力疗法对血管内皮细胞的毒性作用。方法：人脐静脉内皮细胞以等量密度接种于96孔板中培养，按加入ICG的剂量不同分成0、50、100、150、200和250μmol／L组，共6组，每组8孔，其中4孔用波长为810 nm能量密度为24．0 J／cm2的半导体激光照射，另4孔不照射作对照。24 h后用MTT法测量各组细胞的存活率，并对细胞染色镜检。结果：在810 nm的激光照射下，含有ICG的各组内皮细胞的存活率较未照射组显著下降（均 P ＜0），且随着 ICG 浓度的升高，光照组细胞存活率逐渐降低（P ＜0）。当ICG的剂量超过150μmol／L时，细胞的形态变化从凋亡为主转变为坏死为主。结论：适当剂量的ICG介导的光动力疗法对体外培养的血管内皮细胞有较强的抑制作用，有望成为新生血管性疾病的一种新的治疗药物。
목적：연구체외신타정록（ICG）개도적광동력요법대혈관내피세포적독성작용。방법：인제정맥내피세포이등량밀도접충우96공판중배양，안가입ICG적제량불동분성0、50、100、150、200화250μmol／L조，공6조，매조8공，기중4공용파장위810 nm능량밀도위24．0 J／cm2적반도체격광조사，령4공불조사작대조。24 h후용MTT법측량각조세포적존활솔，병대세포염색경검。결과：재810 nm적격광조사하，함유ICG적각조내피세포적존활솔교미조사조현저하강（균 P ＜0），차수착 ICG 농도적승고，광조조세포존활솔축점강저（P ＜0）。당ICG적제량초과150μmol／L시，세포적형태변화종조망위주전변위배사위주。결론：괄당제량적ICG개도적광동력요법대체외배양적혈관내피세포유교강적억제작용，유망성위신생혈관성질병적일충신적치료약물。
Objective To investigate the photo-toxic effects of indocyanine green (ICG) based on photodynamic therapy (PDT) on vascular endothelial cells in vitro. Methods Human umbilical vein endothelial cells were incubated with ICG with different concentrations in 96-well plate. According to the dose of ICG , they were divided into 6 groups as 0, 50, 100, 150, 200 and 250 μmol/L group. Each group consists of 8 wells, 4 of them were irradiated with a 810 nm laser of total power 24.0 J/cm2. Cell viability was measured by MTT assay 24 h after light irradiation. Morphological changes in cells were observed by light microscope. Results With the irradiation of 810 nm light , there was a significant decrease in relative cell viability in all groups except 0 μmol/L group (all P < 0). With the increase of concentration of ICG, the cell viability dropped rapidly (P < 0). Apoptotic changes can be seen in all groups except 0 μmol/L group and necrosis was the main change in cells of 200 μmol/L and 250 μmol/L group. Conclusion PDT with appropriate concentration of ICG has a strong inhibition to vascular endothelial cells in vitro and ICG-PDT may be a good new therapy for nevascularization.