CAJ | 학술논문

目的：通过检测17β－雌二醇在大鼠骨髓间充质干细胞（BMSCs）和不同组织中的表达情况，初步探索非性腺源性雌激素的分泌情况，为进一步研究BMSCs的生理功能以及绝经后骨质疏松的发病机制提供依据。方法：Elisa法检测0、24、48 h时BMSCS培养上清及细胞裂解物中的17β－雌二醇水平。选取SD大鼠不同组织，称重后物理破碎，Elisa法检测不同组织中的17β－雌二醇含量。结果：48 h培养上清中17β－雌二醇浓度较24 h培养上清中明显升高（P＜0．05）。卵巢及软骨组织中17β－雌二醇的浓度高于其他组织，但不具有明显统计学差异（P＞0．05）。结论：大鼠BMSCs可自身合成及分泌17β－雌二醇，其浓度在一定范围内与时间成正比，但对其自身或周围组织是否具有自分泌或旁分泌作用，还有待进一步研究。大鼠不同组织中17β－雌二醇浓度无明显差异，说明其旁分泌现象可能普遍存在且无性腺依赖性，但是否由该被测位点分泌尚需要进一步研究。这种非性腺源性的雌激素可能会成为绝经后参与骨代谢并维持女性骨骼健康的主要雌激素来源。
목적：통과검측17β－자이순재대서골수간충질간세포（BMSCs）화불동조직중적표체정황，초보탐색비성선원성자격소적분비정황，위진일보연구BMSCs적생리공능이급절경후골질소송적발병궤제제공의거。방법：Elisa법검측0、24、48 h시BMSCS배양상청급세포렬해물중적17β－자이순수평。선취SD대서불동조직，칭중후물리파쇄，Elisa법검측불동조직중적17β－자이순함량。결과：48 h배양상청중17β－자이순농도교24 h배양상청중명현승고（P＜0．05）。란소급연골조직중17β－자이순적농도고우기타조직，단불구유명현통계학차이（P＞0．05）。결론：대서BMSCs가자신합성급분비17β－자이순，기농도재일정범위내여시간성정비，단대기자신혹주위조직시부구유자분비혹방분비작용，환유대진일보연구。대서불동조직중17β－자이순농도무명현차이，설명기방분비현상가능보편존재차무성선의뢰성，단시부유해피측위점분비상수요진일보연구。저충비성선원성적자격소가능회성위절경후삼여골대사병유지녀성골격건강적주요자격소래원。
Objective To investigate the concentration of 17β-estradiol in bone marrow stem cells (BMSCs) and different tissues of rats, and study the function of estradiol produced in extragonadal sites prelimi-narily. Methods (1) The concentration of 17β-estradiol in lysate of BMSCs and in supernatant cell culture media were detected by Elisa method after rats BMSCs were serum-free cultured in 0 h , 24 h and 48 h respective ly . (2) The tissues of organs were grinded and broken under ultrasonic wave , then washed and weighed. The con-centration of 17β-estradiol in different tissues was detected by Elisa method. Results Compared with the concen-tration of 17β-estradiol at 24 h, the concentration of 17β-estradiol at 48h significantly increased (P < 0.05). The concentration of 17β-estradiol among different tissues were higher, but the difference is not significant (P > 0.05). Conclusions (1) 17β-estradiol can be secreted by BMSCs , and the concentration is proportional to the time to some degree. But whether the extragonadal estrogen can function locally is still unclear. (2) The concentration of 17β-estradiol is non- gonad dependent but whether it is secreted locally remains to be elucidated. The non- gonad estrogen could be the estrogen source of bone metabolism in order to sustain bone health after menopause.