CAJ | 학술논문

目的：探讨miR-21在1型糖尿病患者外周血单个核细胞（PBMC）分泌干扰素－γ（IFN-γ）与白介素－17（IL-17）的影响。方法：采集60例1型糖尿病患者及60例正常健康人外周血，分离外周血单个核细胞（PBMC），用 qRT-PCR 检测两组 PBMC 中 miR-21的表达；向 PBMC 中转染 miR-21，ELISA 检测 PBMC 分泌IFN-γ与IL-17情况。结果：1型糖尿病患者 PBMC 中 miR-21表达低于对照组，差异有统计学意义（P ＜0．01）；增加 1型糖尿病患者 PBMC 中 miR-21的表达，IFN-γ与 IL-17的分泌均减少，差异有统计学意义（P值分别＜0．05或＜0．01）。结论：miR-21在 1型糖尿病患者 PBMC 中的表达下降，并可抑制 PBMC 中IFN-γ与IL-17的分泌。
목적：탐토miR-21재1형당뇨병환자외주혈단개핵세포（PBMC）분비간우소－γ（IFN-γ）여백개소－17（IL-17）적영향。방법：채집60례1형당뇨병환자급60례정상건강인외주혈，분리외주혈단개핵세포（PBMC），용 qRT-PCR 검측량조 PBMC 중 miR-21적표체；향 PBMC 중전염 miR-21，ELISA 검측 PBMC 분비IFN-γ여IL-17정황。결과：1형당뇨병환자 PBMC 중 miR-21표체저우대조조，차이유통계학의의（P ＜0．01）；증가 1형당뇨병환자 PBMC 중 miR-21적표체，IFN-γ여 IL-17적분비균감소，차이유통계학의의（P치분별＜0．05혹＜0．01）。결론：miR-21재 1형당뇨병환자 PBMC 중적표체하강，병가억제 PBMC 중IFN-γ여IL-17적분비。
Objective To investigate the effect of miR-21 on the secretion of IFN-γ and IL-17 from peripheral blood mononuclear cells (PBMC) in type 1 diabetic patients (T1D). Methods The expression levels of miR-21 in PBMC from 60 T1D patients and controls by quantitative real-time quantitative polymerase chain reaction (qRT-PCR) were detected and the secretions of IFN-γ and IL-17 from T1D PBMC after transfection with miR-21 were detected by enzyme linked immunosorbent assay. Results The expression of miR-21 in PBMC from T1D patients was significantly lower than the control (P<0.01). The levels of IFN-γand IL-17 from T1D PBMC after transfection with miR-21 were remarkably downregulated compared to the control (P < 0.05 or P < 0.01). Conclusion Compared with that at the healthy control group, the expression of miR-21 in PBMC from T1D patients is decreased. MiR-21 could suppress the secretions of IFN-γand IL-17 from PBMC in T1D patients.