CAJ | 학술논문

对哈茨木霉（Trichoderma harzianum GIM 3．442）产β-1，3-葡聚糖内切酶的液体发酵条件进行了单因素优化实验，确定了最佳培养基成分和培养条件，在此基础上通过正交试验设计对复合碳源（葡萄糖、茯苓多糖）、胰蛋白胨、NaNO3和磷酸盐进行了L9（34）试验，研究了4种因素对哈茨木霉产酶的影响，确定了最佳培养条件：葡萄糖42．0 g／L，茯苓多糖18．0 g／L，胰蛋白胨15．0 g／L， NaNO35．0 g／L，初始pH 6．0，接种量8％，28℃，110 r／min培养6 d。优化后总酶活Etotal和β-1，3-葡聚糖内切酶Eendo活力达到了471．6 U／mL和327．4 U／mL，比优化前分别提高了7．3倍和23倍，且内切酶占总酶活的比例Eendo／Etotal由0．24增加到0．71，效果显著。
대합자목매（Trichoderma harzianum GIM 3．442）산β-1，3-포취당내절매적액체발효조건진행료단인소우화실험，학정료최가배양기성분화배양조건，재차기출상통과정교시험설계대복합탄원（포도당、복령다당）、이단백동、NaNO3화린산염진행료L9（34）시험，연구료4충인소대합자목매산매적영향，학정료최가배양조건：포도당42．0 g／L，복령다당18．0 g／L，이단백동15．0 g／L， NaNO35．0 g／L，초시pH 6．0，접충량8％，28℃，110 r／min배양6 d。우화후총매활Etotal화β-1，3-포취당내절매Eendo활력체도료471．6 U／mL화327．4 U／mL，비우화전분별제고료7．3배화23배，차내절매점총매활적비례Eendo／Etotal유0．24증가도0．71，효과현저。
The liquid flask fermentation conditions including medium composition and cultural conditions of Trichoderma harzianum Rifai GIM 3. 442 for producing β-1,3-endoglucanase were optimized. Then the effects of four factors such as composite carbon (glucose,pachymaran),tryptone,NaNO3 and KH2 PO4 were optimized by orthogonal experiment. Re-sults showed that the optimal medium conditions were as follows:glucose 42. 0 g/L,pachymaran 18. 0 g/L,tryptone 15. 0 g/L,NaNO3 5. 0 g/L;initial pH 6. 0,inoculum size 8%,at 28 °C,110 r/min and incubation for 6 d. The total enzyme activity Etotal and β-1,3-endoglucanase activity Eendo reached 471. 6 U/mL and 327. 4 U/mL,increased by 7. 3-fold and 23-fold respectively compared with those of the original activity,and the ratio of β-1,3-endoglucanase in total enzyme activity increased significantly from 0 . 24 to 0 . 71 .