CAJ | 학술논문

分离纯化黄绿蜜环菌菌丝体粗多糖，提高多糖含量，以进一步应用于工业化生产。采用DEAE-52纤维素层析柱分离纯化黄绿蜜环菌菌丝体多糖，依次用水、0．1 mol／L NaCl、0．2 mol／L NaCl、0．4 mol／L NaCl、0．8 mol／L NaCl进行洗脱；苯酚-硫酸法测定纯化后多糖含量，测定波长490 nm，葡萄糖浓度与吸光度的回归方程为A＝6．3137X＋0．2156R2＝0．9909。黄绿蜜环菌菌丝体水体粗多糖经DEAE-52纤维素层析柱分离纯化后，多糖含量达到99％以上。经过中试试验验证提取及分离纯化工艺适用于工业化生产。
분리순화황록밀배균균사체조다당，제고다당함량，이진일보응용우공업화생산。채용DEAE-52섬유소층석주분리순화황록밀배균균사체다당，의차용수、0．1 mol／L NaCl、0．2 mol／L NaCl、0．4 mol／L NaCl、0．8 mol／L NaCl진행세탈；분분-류산법측정순화후다당함량，측정파장490 nm，포도당농도여흡광도적회귀방정위A＝6．3137X＋0．2156R2＝0．9909。황록밀배균균사체수체조다당경DEAE-52섬유소층석주분리순화후，다당함량체도99％이상。경과중시시험험증제취급분리순화공예괄용우공업화생산。
In order to enhance the content of polysaccharide,isolation and purification of polysaccharide of Armillaria luteo-rivens mycelium were conducted for the application to industrial production. The polysaccharide in Armillaria luteo-rivens mycelium was separated and purified by DEAE-52,and eluted gradiently by water,0. 1 mol/L NaCl,0. 2 mol/L NaCl,0. 4 mol/L NaCl and 0. 8 mol/L NaCl;the content of polysaccharide was measured by the phenol-sulfuric acid method,determined with wavelength of 490 nm. The linear relationship of glucose concentration was good with regression equation A=6. 3137X+0. 2156(R2 =0. 9909). The content of polysaccharide exceeded 99% after isolation and purifica-tion. The results showed that the process of extraction,separation and purification was suitable for industrial production.