CAJ | 학술논문

实验室条件下，考察了在发酵过程中不同氮源对小球藻的生物量和油脂积累的影响，确定了小球藻的最佳氮源；并对比分析了含氮培养与缺氮培养的生物量、油脂含量、氮消耗量、生物量氮消耗比率和油脂氮消耗比率的不同。结果表明：小球藻在1．6 g／L NaNO3时获得最大生物量，为562．2 mg／L，在0．8 g／L NaNO3时获得最大相对油脂含量为12．01％；以油脂含量为考察指标时，培养小球藻的最佳氮源为0．8 g／L NaNO3；缺氮培养时，最大油脂含量为13．49％，比含氮培养高约15％；含氮培养时，最高生物量为626．3 mg／L，比缺氮培养高约1．9倍。氮源对生物量，相对油脂含量，生物量氮消耗比率和油脂氮消耗比率具有明显的影响。藉此，提出了通过改变培养方式，达到调控小球藻细胞内生理代谢组分的可行性。
실험실조건하，고찰료재발효과정중불동담원대소구조적생물량화유지적루적영향，학정료소구조적최가담원；병대비분석료함담배양여결담배양적생물량、유지함량、담소모량、생물량담소모비솔화유지담소모비솔적불동。결과표명：소구조재1．6 g／L NaNO3시획득최대생물량，위562．2 mg／L，재0．8 g／L NaNO3시획득최대상대유지함량위12．01％；이유지함량위고찰지표시，배양소구조적최가담원위0．8 g／L NaNO3；결담배양시，최대유지함량위13．49％，비함담배양고약15％；함담배양시，최고생물량위626．3 mg／L，비결담배양고약1．9배。담원대생물량，상대유지함량，생물량담소모비솔화유지담소모비솔구유명현적영향。자차，제출료통과개변배양방식，체도조공소구조세포내생리대사조분적가행성。
The effects of different nitrogen sources on the growth and oil accumulation of Chlorella vulgaris were studied and the optimum nitrogen source was obtained. Meanwhile,the growth,oil accumulation,nitrogen consumption,cell yield coefficient to nitrogen and lipid yield coefficient to nitrogen under the conditions of nitrogen cultivation and nitrogen starvation cultivation were compared and analyzed. The results showed that the maximum biomass was obtained at 1. 6 g/L NaNO3 and the maximum lipid accumulation was obtained at 0. 8 g/L NaNO3;the optimal nitrogen source of Chlorella type and its concentration was 0. 8 g/L NaNO3 . Under the condition of nitrogen starvation cultivation,cultivate chlorella in stages of oil content was 13 . 49%,increased 15% if compared with that of the nitrogen training;the highest biomass was 626. 3 mg/L under single step training,about 1. 9 times higher than that of the nitrogen starvation cultivation. These results collectively suggested that nitrogen sources could obviously affect the biomass,oil content,cell yield coefficient to nitrogen and lipid yield coefficient to nitrogen. Therefore,nitrogen source could be modified to regulate physiological metabolism during the cultivation of Chlorella vulgaris.