CAJ | 학술논문

采用新型常压室温等离子体（ARTP）诱变活跃链霉菌（Streptomyces actuosu），并应用抑菌圈和48孔板培养方法高通量筛选高产那西肽菌株。研究表明抑菌圈径的大小与48孔板效价之间以及48孔板效价与摇瓶效价之间均有较好的相关性，系数R分别达到0．534和0．896。通过多轮ARTP诱变及高通量筛选最终获得了3株相对效价提高50％以上的遗传性能稳定的突变株。AR-TP诱变技术作为获得那西肽高产菌株的有效途径，与传统摇瓶发酵筛选相比，48孔板及抑菌圈法能显著提高那西肽高产菌株的筛选效率。
채용신형상압실온등리자체（ARTP）유변활약련매균（Streptomyces actuosu），병응용억균권화48공판배양방법고통량사선고산나서태균주。연구표명억균권경적대소여48공판효개지간이급48공판효개여요병효개지간균유교호적상관성，계수R분별체도0．534화0．896。통과다륜ARTP유변급고통량사선최종획득료3주상대효개제고50％이상적유전성능은정적돌변주。AR-TP유변기술작위획득나서태고산균주적유효도경，여전통요병발효사선상비，48공판급억균권법능현저제고나서태고산균주적사선효솔。
A novel atmospheric and room temperature plasma (ARTP)mutagenesis system was used by combining with the high throughput screening of 48-well microplates and inhibition zone method to obtain high-yielding mutants. The results showed that the correlation coefficients between the ratio of inhibition zone diameter and mA408 ,and mA408 between fA408 reached 0. 534 and 0. 896,respectively. Through several rounds of ARTP mutation and high-throughput screening, three genetic stable mutants were obtained with increase by 50% relative contents of nosiheptide than that of the control strain finally. ARTP mutagenesis techniques as an effective way to obtain high-yield nosiheptide strains was compared with the traditional shake flask screening method,48-well microplate and inhibition zone method could significantly improve the screening efficiency of high nosiheptide-producing strains.