CAJ | 학술논문

采用生物法，利用重组葡萄糖苷酶催化芦荟苷合成芦荟大黄素，通过单因素试验及均匀试验设计得到了合成芦荟大黄素的最佳工艺条件：反应时间4 h，酶浓度50 U/ mL，叔丁醇：水（v/ v）=5：5，反应温度43℃，pH 值5.2.该工艺条件下芦荟大黄素转化率为92.18%.
채용생물법，이용중조포도당감매최화호회감합성호회대황소，통과단인소시험급균균시험설계득도료합성호회대황소적최가공예조건：반응시간4 h，매농도50 U/ mL，숙정순：수（v/ v）=5：5，반응온도43℃，pH 치5.2.해공예조건하호회대황소전화솔위92.18%.
Recombinant glucosidase was used to catalysis biosynthesize of aloe-emodin through aloin. Single factor experiment and uniform design were used to optimize the biosynthesize conditions. As the result,the optimal conditons of aleo-emodin’s biosynthesis were as following:reaction time 4 h,combinant glucosidase concentration 50 U/ mL,the ratio of tert-butanol and water 5: 5(v/ v),reaction temperature 43 ℃ ,and pH value 5. 2. Under those conditions,the highest yield was 92. 18% .