CAJ | 학술논문

目的：观察半夏泻心汤对N-甲基-N′-硝基-N-亚硝基胍( N-methyl-N′-nitro-N-nitrosog-uanidine,MNNG诱导大鼠前胃鳞癌的防治作用。方法将140只雄性SD大鼠随机分为3组,空白组20只、模型组100只、模型中药组20只,采用MNNG联合多因素造模。每4周抽检模型组大鼠4只,观察前胃黏膜组织学变化,确定造模是否成功。造模成功后抽检各组大鼠,并将剩余模型组39只大鼠随机分为4组,模型对照组9只,半夏泻心汤高、中、低剂量组各10只,分别给予0.9%生理盐水、2.29、1.375、0.6875 g/mL的半夏泻心汤灌胃,10 mL/kg,1次/天,连续12周。40周末,处死全部大鼠,观察前胃黏膜组织学变化。结果28周末前胃鳞状上皮增生、异型增生、鳞癌发生率,空白组为5%、5%、0,模型组为100%、83.3%、50%,模型中药组为0、0、0。40周末前胃鳞状上皮增生、异型增生、鳞癌发生率,模型对照组为100%、100%、77.8%,高剂量组为100%、85.7%、57.1%,中剂量组为100%、44.5%、22.3%,低剂量组为100%、77.8%、66.7%。结论半夏泻心汤对MNNG多因素造模法诱发大鼠前胃鳞癌的发生具有一定的防治作用。
목적：관찰반하사심탕대N-갑기-N′-초기-N-아초기고( N-methyl-N′-nitro-N-nitrosog-uanidine,MNNG유도대서전위린암적방치작용。방법장140지웅성SD대서수궤분위3조,공백조20지、모형조100지、모형중약조20지,채용MNNG연합다인소조모。매4주추검모형조대서4지,관찰전위점막조직학변화,학정조모시부성공。조모성공후추검각조대서,병장잉여모형조39지대서수궤분위4조,모형대조조9지,반하사심탕고、중、저제량조각10지,분별급여0.9%생리염수、2.29、1.375、0.6875 g/mL적반하사심탕관위,10 mL/kg,1차/천,련속12주。40주말,처사전부대서,관찰전위점막조직학변화。결과28주말전위린상상피증생、이형증생、린암발생솔,공백조위5%、5%、0,모형조위100%、83.3%、50%,모형중약조위0、0、0。40주말전위린상상피증생、이형증생、린암발생솔,모형대조조위100%、100%、77.8%,고제량조위100%、85.7%、57.1%,중제량조위100%、44.5%、22.3%,저제량조위100%、77.8%、66.7%。결론반하사심탕대MNNG다인소조모법유발대서전위린암적발생구유일정적방치작용。
Objective To observe the preventive and therapeutic effects of Banxia Xiexin Decoc-tion on rats with forestomach squamous carcinoma induced by MNNG. Methods 140 SD rats were ran-domly divided into 3 groups:control group (20 rats), model group (100 rats), and model and tradition-al Chinese medicine (TCM) group (20 rats). The rat models were established with rats in all the groups except control group by using combination MNNG and multiple factors methods. 4 rats were randomly tested by observing on the histological changes of forestomach mucosa every 4 weeks, in order to deter-mine whether the rat models establishing was successful. After rat models establishment, there were 39 rats left in model group, and the rats were randomly divided into 4 groups:model control group (9 rats, received gavage administration with normal saline) , high, middle and low dosage of Banxia Xiexin De-coction group ( with 10 rats in each group, received gavage administration with Banxia Xiexin Decoction with dosage of 2. 29 g/mL, 1. 375 g/mL, and 0. 6875 g/mL). The volume of gavage administration was 10 mL/(kg·d), and the treatment lasted for 12 weeks. At the end of the 40th week, all the rats were sacrificed, in order to observe the histological changes of forestomach mucosa. Results At the end of the 28th week, the incidence rate of squamous epithelium hyperplasia, atypical hyperplasia and squa-mous carcinoma in forestomach was 5%, 5% and 0 respectively of rats in control group, model group was 100%, 83. 3% and 50% respectively, while model and TCM group was 0, 0 and 0 respectively. At the end of the 40th week, the incidence rate of squamous epithelium hyperplasia, atypical hyperplasia and squamous carcinoma in forestomach of rats in model control group was 100%, 100% and 77. 8% respec-tively, and these of rats in high dosage of Banxia Xiexin group was 100%, 85. 7% and 57. 1%, middle dosage of Banxia Xiexin group was 100%, 44. 5% and 22. 3%, and low dosage of Banxia Xiexin group was 100%, 77. 8% and 66. 7%. Conclusion There was a certain preventive and therapeutic effects of Banxia Xiexin decoction on rats with forestomach squamous carcinoma induced by MNNG.