针灸临床杂志
針灸臨床雜誌
침구림상잡지
JOURNAL OF CLINICAL ACUPUNCTURE AND MOXIBUSTION
2015年
4期
59-63
,共5页
罗钦%李胜杰%刘文吉%张伟%冀美琦%解秸萍
囉欽%李勝傑%劉文吉%張偉%冀美琦%解秸萍
라흠%리성걸%류문길%장위%기미기%해갈평
电针%天灸%肥大细胞%肝纤维化%大鼠
電針%天灸%肥大細胞%肝纖維化%大鼠
전침%천구%비대세포%간섬유화%대서
Electro-acupuncture%Medicinal vesiculation%Mast cells%Hepatic fibrosis%Rats
目的：探讨电针与天灸对肝纤维化（ HF）大鼠肝俞穴区皮肤肥大细胞（ MCs）的影响差异。方法：雄性SD大鼠随机分为正常组、模型组、电针组和天灸组，每组7只。腹腔注射CCl4制备HF模型，第3周起，电针组、天灸组分别予肝俞穴电针刺激、斑蝥贴敷，共4周。治疗结束次日，取左侧肝俞穴区备皮，甲苯胺蓝染色，分层次观察MCs及其脱颗粒情况。结果：穴区MCs总数及其脱颗粒数（率）：①MCs总数：模型组与正常组间无明显差异，电针组和天灸组较模型组显著增多（P＜0．01），电针组与天灸组间无显著差异；②MCs脱颗粒数：模型组与正常组、电针组与模型组间无显著差异，天灸组较模型组、电针组显著增多（ P＜0．01或P＜0．05）；③MCs脱颗粒率：模型组、电针组和天灸组较正常组显著升高（ P＜0．05或P＜0．01），但3组间无显著差异。穴区皮肤各层MCs分布：①组内比较：各组皮下组织层、真皮网状层MCs总数，正常组、模型组皮下组织层、真皮网状层MCs脱颗粒数，电针组皮下组织层和天灸组真皮网状层脱颗粒数均显著高于真皮乳头层（P＜0．05或P＜0．01）；正常组、模型组皮下组织层MCs总数、电针皮下组织层MCs总数和脱颗粒数均显著高于真皮网状层（P＜0．01），天灸组真皮网状层脱颗粒数显著高于皮下组织层（ P＜0．01）。②组间比较：模型组与正常组各层MCs总数、脱颗粒数分布无明显差异；真皮乳头层与真皮网状层：天灸组此两层MCs总数、脱颗粒数均较模型组、电针组显著升高（P＜0．05或P＜0．01）；皮下组织层：MCs总数电针组、天灸组较模型组均显著升高（P＜0．01或P＜0.05），电针组与天灸组间无显著差异；MCs脱颗粒数电针组、天灸组较模型组均无显著性差异，但电针组较正常组、天灸组显著升高（P＜0．01或P＜0．05）。结论：电针与天灸对穴区MCs均有募集、激活作用，天灸作用更为显著；电针与天灸可引起穴区皮肤MCs的趋向性分布，电针主要集中在皮下组织层，天灸为真皮乳头层和真皮网状层。
目的：探討電針與天灸對肝纖維化（ HF）大鼠肝俞穴區皮膚肥大細胞（ MCs）的影響差異。方法：雄性SD大鼠隨機分為正常組、模型組、電針組和天灸組，每組7隻。腹腔註射CCl4製備HF模型，第3週起，電針組、天灸組分彆予肝俞穴電針刺激、斑蝥貼敷，共4週。治療結束次日，取左側肝俞穴區備皮，甲苯胺藍染色，分層次觀察MCs及其脫顆粒情況。結果：穴區MCs總數及其脫顆粒數（率）：①MCs總數：模型組與正常組間無明顯差異，電針組和天灸組較模型組顯著增多（P＜0．01），電針組與天灸組間無顯著差異；②MCs脫顆粒數：模型組與正常組、電針組與模型組間無顯著差異，天灸組較模型組、電針組顯著增多（ P＜0．01或P＜0．05）；③MCs脫顆粒率：模型組、電針組和天灸組較正常組顯著升高（ P＜0．05或P＜0．01），但3組間無顯著差異。穴區皮膚各層MCs分佈：①組內比較：各組皮下組織層、真皮網狀層MCs總數，正常組、模型組皮下組織層、真皮網狀層MCs脫顆粒數，電針組皮下組織層和天灸組真皮網狀層脫顆粒數均顯著高于真皮乳頭層（P＜0．05或P＜0．01）；正常組、模型組皮下組織層MCs總數、電針皮下組織層MCs總數和脫顆粒數均顯著高于真皮網狀層（P＜0．01），天灸組真皮網狀層脫顆粒數顯著高于皮下組織層（ P＜0．01）。②組間比較：模型組與正常組各層MCs總數、脫顆粒數分佈無明顯差異；真皮乳頭層與真皮網狀層：天灸組此兩層MCs總數、脫顆粒數均較模型組、電針組顯著升高（P＜0．05或P＜0．01）；皮下組織層：MCs總數電針組、天灸組較模型組均顯著升高（P＜0．01或P＜0.05），電針組與天灸組間無顯著差異；MCs脫顆粒數電針組、天灸組較模型組均無顯著性差異，但電針組較正常組、天灸組顯著升高（P＜0．01或P＜0．05）。結論：電針與天灸對穴區MCs均有募集、激活作用，天灸作用更為顯著；電針與天灸可引起穴區皮膚MCs的趨嚮性分佈，電針主要集中在皮下組織層，天灸為真皮乳頭層和真皮網狀層。
목적：탐토전침여천구대간섬유화（ HF）대서간유혈구피부비대세포（ MCs）적영향차이。방법：웅성SD대서수궤분위정상조、모형조、전침조화천구조，매조7지。복강주사CCl4제비HF모형，제3주기，전침조、천구조분별여간유혈전침자격、반모첩부，공4주。치료결속차일，취좌측간유혈구비피，갑분알람염색，분층차관찰MCs급기탈과립정황。결과：혈구MCs총수급기탈과립수（솔）：①MCs총수：모형조여정상조간무명현차이，전침조화천구조교모형조현저증다（P＜0．01），전침조여천구조간무현저차이；②MCs탈과립수：모형조여정상조、전침조여모형조간무현저차이，천구조교모형조、전침조현저증다（ P＜0．01혹P＜0．05）；③MCs탈과립솔：모형조、전침조화천구조교정상조현저승고（ P＜0．05혹P＜0．01），단3조간무현저차이。혈구피부각층MCs분포：①조내비교：각조피하조직층、진피망상층MCs총수，정상조、모형조피하조직층、진피망상층MCs탈과립수，전침조피하조직층화천구조진피망상층탈과립수균현저고우진피유두층（P＜0．05혹P＜0．01）；정상조、모형조피하조직층MCs총수、전침피하조직층MCs총수화탈과립수균현저고우진피망상층（P＜0．01），천구조진피망상층탈과립수현저고우피하조직층（ P＜0．01）。②조간비교：모형조여정상조각층MCs총수、탈과립수분포무명현차이；진피유두층여진피망상층：천구조차량층MCs총수、탈과립수균교모형조、전침조현저승고（P＜0．05혹P＜0．01）；피하조직층：MCs총수전침조、천구조교모형조균현저승고（P＜0．01혹P＜0.05），전침조여천구조간무현저차이；MCs탈과립수전침조、천구조교모형조균무현저성차이，단전침조교정상조、천구조현저승고（P＜0．01혹P＜0．05）。결론：전침여천구대혈구MCs균유모집、격활작용，천구작용경위현저；전침여천구가인기혈구피부MCs적추향성분포，전침주요집중재피하조직층，천구위진피유두층화진피망상층。
Objective:To compare and discuss the different effects of electro-acupuncture ( EA) and medici-nal vesiculation ( MV) on mast cells ( MCs) activation in BL18 acupoint areas of hepatic fibrosis ( HF) rats. Methods:28 male SD rats were randomized into normal, model, EA and MV groups (7rats/group) .The HF model was made by intraperitoneal injection with CCl4 (40%CCl4 mixed with olive oil, 0.3 ml/100 g per rat, twice per week for 6 weeks) .From the third week, EA group received EA on BL18 (1 mA, 50 Hz, 20 min, every other day for 4weeks), and MV group received BL18 point-application with cantharides (0.05 g can-tharides mixed water into paste, 6hours, twice per week for 4weeks).On the next day after finishing all treat-ments, the left-hand skin tissues of BL18 were sampled, stained with toluidine blue for the observation of MCs activation in different layers.Results:Cutaneous MCs of BL18 total number and degranulated number( rate):①MCs total number:No obvious difference was shown between model group and normal group;EA group and MV group were significantly increased than model group ( P<0 .01 ); no significant difference was shown be-tween EA group and MV group;②MCs degranulated number: No significant differences were shown between model group and normal group and between EA group and model group;MV group was much higher than model group and EA group (P<0.01 or P<0.05);③MCs degranulated rate:Compared with normal group, model group, EA group and MV group were all significantly raised(P<0.05 or P<0.01), and no obvious differ-ences were shown among the three groups.MCs distribution in each cutaneous layer of BL18: ①Comparison within each group:MCs total numbers of subcutaneous layer and dermal reticular layer in each group,and MCs degranulated numbers of subcutaneous layer and dermal reticular layer in normal group and model group, MCs degranulated numbers of subcutaneous layer in EA group and dermal reticular layer in MV group were all signif -icantly increased than those in dermal papilla layer(P<0.05 or P<0.01);MCs total numbers of subcutaneous layer in normal group and model group, MCs total and degranulated numbers of subcutaneous layer in EA group were much higher than those in dermal reticular layer( P<0.01) ,and MCs degranulated number of dermal lay-er in MV group was obviously higher than that in subcutaneous layer(P<0.01).②Comparison among groups:Between normal group and model group, the MCs total and degranulated numbers had no obvious differences in each layer;the dermal papilla layer and reticular layer:The MCs total and degranulated numbers of MV group in the two layers were substantially higher than those of model group and EA group(P<0.05 or P<0.01); the subcutaneous layer:MCs total number: EA group and MV group were markedly increased than model group (P<0.01 or P<0.05), and there was no obvious difference between EA group and MV group, MCs degranu-lated number:Compared with model group, no significant differences were shown in EA group and MV group, while EA group was much higher than normal group and MV group( P<0.01 or P<0.05) .Conclusion:Both EA and MV can recruit and activate MCs in acupoint areas and MV shows a stronger effect; EA and MV can adjust the distribution of MCs in acupoint areas.EA can make MCs gathered in the subcutaneous layer and MV in the dermal papilla layer and dermal reticular layer.
