河北中医
河北中醫
하북중의
HEBEI JORNAL OF TRADITIONAL CHINESE MEDICINE
2015年
4期
550-552,586
,共4页
赵智明%常文静%赵凌杰%董晓蕾%沈思钰%蔡辉
趙智明%常文靜%趙凌傑%董曉蕾%瀋思鈺%蔡輝
조지명%상문정%조릉걸%동효뢰%침사옥%채휘
心肌疾病%胶原%心室重构%疾病模型,动物%模型,动物%动物实验%大鼠%丹参酮%丹参酮ⅡA%压力负荷%心肌羟脯氨酸
心肌疾病%膠原%心室重構%疾病模型,動物%模型,動物%動物實驗%大鼠%丹參酮%丹參酮ⅡA%壓力負荷%心肌羥脯氨痠
심기질병%효원%심실중구%질병모형,동물%모형,동물%동물실험%대서%단삼동%단삼동ⅡA%압력부하%심기간포안산
Myocardial diseases%Collagen%Ventricular remodeling%Disease model%Animal%Animal model%Animal experiment%Rat%Tanshinone%Tanshinone ⅡA
目的:观察丹参酮ⅡA对压力负荷增加大鼠心室胶原重构的影响。方法采用腹主动脉缩窄术制备压力负荷增加大鼠模型,动脉夹闭(其中8只大鼠不结扎腹主动脉,为假手术组)4周后,分为5组:假手术组、模型组、丹参酮ⅡA低剂量组[10 mg/(kg· d)]、丹参酮ⅡA高剂量组[20 mg/(kg· d)]及卡托普利组[100 mg/(kg· d)]组,每组各8只,各组均予相应药液腹腔注射(卡托普利组为灌胃给药),连续给药4周后检测各组大鼠心脏质量指数(HWI)和左心室质量指数(LVWI),苏木精—伊红(HE)染色法观察左心室心肌病理学改变,Masson染色法观察左心室心肌胶原形态,化学比色法测定心肌羟脯氨酸( HYP)含量。结果与假手术组比较,模型组大鼠HWI、LVWI、心肌组织HYP含量均明显升高(P<0.05,P<0.01),心肌有不同程度的损害;与模型组比较,卡托普利组、丹参酮ⅡA高剂量组及丹参酮ⅡA低剂量组HWI、LVWI及HYP均明显下降(P<0.01,P<0.05),心肌损害均有不同程度改善。结论丹参酮ⅡA能够抑制压力负荷增加大鼠心肌胶原沉积和心肌HYP的表达,在一定程度可改善心室胶原重构。
目的:觀察丹參酮ⅡA對壓力負荷增加大鼠心室膠原重構的影響。方法採用腹主動脈縮窄術製備壓力負荷增加大鼠模型,動脈夾閉(其中8隻大鼠不結扎腹主動脈,為假手術組)4週後,分為5組:假手術組、模型組、丹參酮ⅡA低劑量組[10 mg/(kg· d)]、丹參酮ⅡA高劑量組[20 mg/(kg· d)]及卡託普利組[100 mg/(kg· d)]組,每組各8隻,各組均予相應藥液腹腔註射(卡託普利組為灌胃給藥),連續給藥4週後檢測各組大鼠心髒質量指數(HWI)和左心室質量指數(LVWI),囌木精—伊紅(HE)染色法觀察左心室心肌病理學改變,Masson染色法觀察左心室心肌膠原形態,化學比色法測定心肌羥脯氨痠( HYP)含量。結果與假手術組比較,模型組大鼠HWI、LVWI、心肌組織HYP含量均明顯升高(P<0.05,P<0.01),心肌有不同程度的損害;與模型組比較,卡託普利組、丹參酮ⅡA高劑量組及丹參酮ⅡA低劑量組HWI、LVWI及HYP均明顯下降(P<0.01,P<0.05),心肌損害均有不同程度改善。結論丹參酮ⅡA能夠抑製壓力負荷增加大鼠心肌膠原沉積和心肌HYP的錶達,在一定程度可改善心室膠原重構。
목적:관찰단삼동ⅡA대압력부하증가대서심실효원중구적영향。방법채용복주동맥축착술제비압력부하증가대서모형,동맥협폐(기중8지대서불결찰복주동맥,위가수술조)4주후,분위5조:가수술조、모형조、단삼동ⅡA저제량조[10 mg/(kg· d)]、단삼동ⅡA고제량조[20 mg/(kg· d)]급잡탁보리조[100 mg/(kg· d)]조,매조각8지,각조균여상응약액복강주사(잡탁보리조위관위급약),련속급약4주후검측각조대서심장질량지수(HWI)화좌심실질량지수(LVWI),소목정—이홍(HE)염색법관찰좌심실심기병이학개변,Masson염색법관찰좌심실심기효원형태,화학비색법측정심기간포안산( HYP)함량。결과여가수술조비교,모형조대서HWI、LVWI、심기조직HYP함량균명현승고(P<0.05,P<0.01),심기유불동정도적손해;여모형조비교,잡탁보리조、단삼동ⅡA고제량조급단삼동ⅡA저제량조HWI、LVWI급HYP균명현하강(P<0.01,P<0.05),심기손해균유불동정도개선。결론단삼동ⅡA능구억제압력부하증가대서심기효원침적화심기HYP적표체,재일정정도가개선심실효원중구。
Objective To observe the effects of tanshinoneⅡA ( TanⅡA) on ventricular collagen remode-ling in rats with pressure overload increased.Methods The myocardial fibrosis rats with pressure overload increased were prepared with abdominal aortic constriction.These rats received abdominal aorta ligation for 4 weeks ( 8 rats of them didn't ligate abdominal aorta, as sham operation group) .The rats were divided into five groups:sham operation group, model group, TanⅡA of low dose group [ L-TanⅡA group,10 mg/( kg· d) ] , TanⅡA of high dose group [H-TanⅡA group, 20 mg/(kg· d)] and captopril group[100 mg/(kg· d)],8 rats in each group.The corre-sponding medicine in each group was given by intraperitoneal injection( captopril group was given by intragastric ad-ministration) , successive administration 4 weeks.After 8 weeks of surgery, heart mass index ( HMI) and left ventric-ular mass index ( LVMI) were detected, left ventricular myocardial histology were observed by HE staining, as well as left ventricular myocardial collagen forms by Masson staining, and ELISA for myocardial hydroxyproline ( HYP) content.Results HMI, LVMI and myocardial HYP content in model group were significantly higher than those in sham operation group(P<0.05,P<0.01), and there were myocardial damage at different degree.Compared with model group, HMI, LVMI and myocardial HYP content in other three groups were obviously decreased (P<0.05,P<0.01), and there were improvement of myocardial damage at different degree.Conclusion TanshinoneⅡA can markedly inhibit myocardial collagen deposition and myocardial expression of HYP in pressure overload increased rats, and improve ventricular collagen remodeling in some extent.