CAJ | 학술논문

目的：比较藏红花有效成分含量及影响因素，为藏红花高产优质栽培与优良种质选育提供依据。方法在2010年版药典与现有文献基础上，优化藏红花有效成分含量HPLC测定方法，并测量比较11个产地藏红花中有效成分含量，用DPS软件分析经纬度、海拔与有效成分含量间的相关性。结果西红花苷-玉和西红花苷-域分别在6.236耀62.356μg/mL以及3.158耀31.584μg/mL范围内与各自峰面积积分值呈良好的线性关系，R2分别为0.9997与0.9996，两者平均回收率分别为99.9%（RSD=1.69%，n=9）与99.4%（RSD=0.86%，n=9）；11个产地藏红花中西红花苷-玉、西红花苷-域、西红花总苷含量存在显著差异（P<0.05，P<0.01）；经度与西红花有效成分含量呈正相关关系，海拔、纬度与西红花有效成分含量呈负相关关系。结论本方法测定藏红花有效成分含量简便、准确、重复性好，我国引种的藏红花有效成分含量高，质量好，低纬度、高经度地区并视种质选择海拔高度区别种植藏红花有利于有效成分积累。
목적：비교장홍화유효성분함량급영향인소，위장홍화고산우질재배여우량충질선육제공의거。방법재2010년판약전여현유문헌기출상，우화장홍화유효성분함량HPLC측정방법，병측량비교11개산지장홍화중유효성분함량，용DPS연건분석경위도、해발여유효성분함량간적상관성。결과서홍화감-옥화서홍화감-역분별재6.236요62.356μg/mL이급3.158요31.584μg/mL범위내여각자봉면적적분치정량호적선성관계，R2분별위0.9997여0.9996，량자평균회수솔분별위99.9%（RSD=1.69%，n=9）여99.4%（RSD=0.86%，n=9）；11개산지장홍화중서홍화감-옥、서홍화감-역、서홍화총감함량존재현저차이（P<0.05，P<0.01）；경도여서홍화유효성분함량정정상관관계，해발、위도여서홍화유효성분함량정부상관관계。결론본방법측정장홍화유효성분함량간편、준학、중복성호，아국인충적장홍화유효성분함량고，질량호，저위도、고경도지구병시충질선택해발고도구별충식장홍화유리우유효성분적루。
Objective To detect the active component contents in Crocus sativus and investigate the influence factor on them, in order to provide scientific basis for high yield and quality cultivation of Crocus sativus and se-lection of good germ plasm. Methods Based on China Pharmacopeia published in 2010 and the existing evidence, the HPLC method for determination of active component contents in Crocus sativus was optimized. The active com-ponent contents in Crocus sativus planted in 11 different producing area were determined and compared. DPS soft-ware was used to find the relationship between latitude, longitude, height, and the active components. Results The linear ranges of crorin-I and crorin-II were 6.236-62.356 μg/mL and 3.158-31.584 μg/mL, the R2 were 0.9997 and 0.9996, and the average recovery rates were 99.9%(RSD=1.69%, n=9) and 99.4%(RSD=0.86%, n=9), respec-tively. Significant difference in crorin-I, crorin-II, and total contents was found between Crocus sativus produced in different areas(P<0.05 or P<0.01). A positive correlation was seen between active component contents and the lon-gitude of producing area; a negative correlation was seen between active component contents and the latitude and height of producing area. Conclusion The optimized HPLC method for determining the active component contents in Crocus sativus is convenient, accurate, and repeatable. The quality of Crocus sativus cultivated in China is high. Cultivation of Crocus sativus in areas with low latitude, high longitude and varied heights according to different germplasm will be benefit.