CAJ | 학술논문

本文将数字全息与显微成像技术相结合，设计搭建了一套数字全息显微系统，用于对浮游生物进行光场获取。基于该系统获取的光场信息，通过在不同景深对图像进行再现，既可以获到单一浮游生物的清晰图像，也可以获得一定水体内浮游生物微粒在海水中的三维分布情况。通过实验测得系统分辨率可以达到7．8微米，景深可以达到10毫米，优于一般光学显微镜的技术指标。研究结果表明优势明显的数字全息显微系统是一种适合海洋原位探测的浮游生物研究的有效方法。基于数字同轴显微系统的水下仪器开发将是下一步工作的努力方向。
본문장수자전식여현미성상기술상결합，설계탑건료일투수자전식현미계통，용우대부유생물진행광장획취。기우해계통획취적광장신식，통과재불동경심대도상진행재현，기가이획도단일부유생물적청석도상，야가이획득일정수체내부유생물미립재해수중적삼유분포정황。통과실험측득계통분변솔가이체도7．8미미，경심가이체도10호미，우우일반광학현미경적기술지표。연구결과표명우세명현적수자전식현미계통시일충괄합해양원위탐측적부유생물연구적유효방법。기우수자동축현미계통적수하의기개발장시하일보공작적노력방향。
In this paper,a new approach of digital holography and microscopy technology had been combined to take images of plankton without intrusion and interference.Digital In-line Holographic Microscopy (DIHM)system was de-signed,not only to record holographic images of the plankton fields but also to reconstruct the tomographic images at dif-ferent depth fields for the use of 3D image analysis.The obtained results demonstrated that the minimum size of the ob-ject that can be clearly imaged with the DIHMsystem was 7.8 μm with the field depth of 10 mm,which was much larger than those an optical microscope could ever achieve.Favorable results from the experiments have shown that DIHMis an appropriate and highly effective method for aquatic plankton study.An in-situ underwater DIHM instrument is hoped to be developed for field study of marine plankton in near future.