CAJ | 학술논문

目的：对乙肝患者血清 HBV-DNA 与前 S1抗原检测的方法进行探讨，了解检测 HBV-DNA 及前 S1抗原在诊断乙肝中的作用。方法选取85例乙型肝炎患者，抽取患者的肘静脉血清，采用荧光定量聚合酶链反应（FQ-PCR）进行 HBV-DNA 检测，使用酶联免疫吸附法（ELISA）对患者的乙肝“两对半”、前 S1抗原进行检测，并对两种方法的检测结果进行统计学分析。结果乙肝患者经过检测后，在不同乙肝“两对半”的模式下，乙肝患者的 HBV-DNA 以及前 S1抗原的阳性率与 HBV-DNA 的阳性率差异并无统计学意义（P ＞0.05）。结论在对乙肝患者进行检测时，两种方法检测的结果具有一致性、有效性，必要时采用两种方法进行联合检测，能够为乙肝患者的临床疗效考核与预后治疗提供必要的实验室数据。
목적：대을간환자혈청 HBV-DNA 여전 S1항원검측적방법진행탐토，료해검측 HBV-DNA 급전 S1항원재진단을간중적작용。방법선취85례을형간염환자，추취환자적주정맥혈청，채용형광정량취합매련반응（FQ-PCR）진행 HBV-DNA 검측，사용매련면역흡부법（ELISA）대환자적을간“량대반”、전 S1항원진행검측，병대량충방법적검측결과진행통계학분석。결과을간환자경과검측후，재불동을간“량대반”적모식하，을간환자적 HBV-DNA 이급전 S1항원적양성솔여 HBV-DNA 적양성솔차이병무통계학의의（P ＞0.05）。결론재대을간환자진행검측시，량충방법검측적결과구유일치성、유효성，필요시채용량충방법진행연합검측，능구위을간환자적림상료효고핵여예후치료제공필요적실험실수거。
Objective To understand the effect of detecting HBV-DNA and Pre-S1 antigen in the diagnosis of hepatitis B patients through probing into the detection methods of the HBV-DNA and Pre-S1 antigen of hepatitis B patients’serum. Methods Selected 85 cases of hepatitis B patients,extract cubital vein serum of the patients,then detected HBV-DNA with fluorescence quantitative polymerase chain reaction(FQ-PCR),while detected“two pairs of semi-hepatitis B”and Pre-S1 antigen with enzyme-linked immunosorbent assay(ELISA),and the detection results of two methods were statistically analyzed. Results After detection,the positive rate difference between HBV-DNA along with Pre-S1 antigen and HBV-DNA of hepatitis B patients was not statistically significant(P > 0.05)in different modes of“two pairs of semi-hepatitis B”. Conclusion In detecting hepatitis B patients,the results of the two methods are consistent and effective. Two methods should be combined to make the detection when necessary,thus providing necessary laboratory data for clinical efficacy assessment and prognosis treatment of the hepatitis B patients.