CAJ | 학술논문

采用超临界CO2萃取技术提取油橄榄叶,提取液经硅胶柱层析分离得到质量分数为96%以上的单体,通过液质联用分析( HPLC-MS)、核磁共振(1H NMR)和红外( IR)鉴定该组分为橄榄苦苷。采用C18色谱柱,紫外检测波长为254 nm,甲醇/水体积比40∶60为流动相,流速0.5 mL/min,建立橄榄苦苷的HPLC分析方法。通过单因素和正交试验研究,得到萃取优化条件为：萃取压力25 MPa,萃取温度45℃,分离温度45℃,萃取时间4 h,CO2流量30 kg/h。在此条件下,橄榄苦苷得率为0.435%。
채용초림계CO2췌취기술제취유감람협,제취액경규효주층석분리득도질량분수위96%이상적단체,통과액질련용분석( HPLC-MS)、핵자공진(1H NMR)화홍외( IR)감정해조분위감람고감。채용C18색보주,자외검측파장위254 nm,갑순/수체적비40∶60위류동상,류속0.5 mL/min,건립감람고감적HPLC분석방법。통과단인소화정교시험연구,득도췌취우화조건위：췌취압력25 MPa,췌취온도45℃,분리온도45℃,췌취시간4 h,CO2류량30 kg/h。재차조건하,감람고감득솔위0.435%。
The Olea europaea leaves were extracted by supercritical carbon dioxide extraction technology, then the extract was analyzed by silica gel column chromatography. A compound was isolated from the extract with content of 96% which was identified as oleuropein by HPLC-MS, 1H NMR, and IR. The HPLC analysis was carried out with column C18 and MeOH-water 40∶60 ( volume ratio) as mobile phase at flow rate 0. 5 mL/min, and monitored at 254 nm. By single factor and orthogonal experiments, optimal extraction conditions were obtained as follows: extraction pressure 25 MPa, extraction temperature 45 ℃, separation temperature 45 ℃,extraction time 4 h, and CO2 flow rate 30 kg/h. At the optimal extraction conditions, the yield of oleuropein was 0. 435%.