现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2015年
2期
16-18
,共3页
孙灵迪%梅传忠%王平均%武晓茜%邵先安
孫靈迪%梅傳忠%王平均%武曉茜%邵先安
손령적%매전충%왕평균%무효천%소선안
聚合酶链反应-直接测序法%磁珠酶联免疫法%人类白细胞抗原B27%强直性脊柱炎
聚閤酶鏈反應-直接測序法%磁珠酶聯免疫法%人類白細胞抗原B27%彊直性脊柱炎
취합매련반응-직접측서법%자주매련면역법%인류백세포항원B27%강직성척주염
polymerase chain reaction sequence-based typing(PCR-SBT)%immunomagnetic separation and enzyme-linked im-munosorbent assay(IMS-ELISA)%human leukocyte antigen B27(HLA B27)%ankylosing spondylitis(AS)
目的:比较聚合酶链反应-直接测序法(polymerase chain reaction sequence-based typing,PCR-SBT)和磁珠酶联免疫法(immunomagnetic separation and enzyme-linked immunosorbent assay,IMS-ELISA)检测人类白细胞抗原 B27(human leukocyte antigen B27,HLA-B27)在强直性脊柱炎(ankylosing spondylitis,AS)临床诊断中的价值。方法应用 PCR-SBT法和 IMS-ELISA 法对120例疑似 AS 患者外周血进行 HLA-B27检测。以 SPSS17.0软件的配对χ2检验和受试者工作特征曲线下面积对两种方法检测 HLA-B27在 AS 诊断中的价值进行评价。结果120例疑似 AS 患者中,PCR-SBT 和 IMS-ELISA 法 HLA-B27检测阳性率分别为45.83%(55/120)和37.50%(45/120)。两种方法检测 HLA-B27差异有统计学意义(χ2=59.455,P =0.000)。PCR-SBT 法敏感度和特异度分别为96.36%和96.92%;IMS-ELISA 法的敏感度和特异度分别为69.09%和89.23%。两者的 AUC 分别为0.966和0.792。结论与 IMS-ELISA 法相比,在 AS 的临床诊断中PCR-SBT 法检测 HLA-B27的敏感度和特异度更高,方法更优。
目的:比較聚閤酶鏈反應-直接測序法(polymerase chain reaction sequence-based typing,PCR-SBT)和磁珠酶聯免疫法(immunomagnetic separation and enzyme-linked immunosorbent assay,IMS-ELISA)檢測人類白細胞抗原 B27(human leukocyte antigen B27,HLA-B27)在彊直性脊柱炎(ankylosing spondylitis,AS)臨床診斷中的價值。方法應用 PCR-SBT法和 IMS-ELISA 法對120例疑似 AS 患者外週血進行 HLA-B27檢測。以 SPSS17.0軟件的配對χ2檢驗和受試者工作特徵麯線下麵積對兩種方法檢測 HLA-B27在 AS 診斷中的價值進行評價。結果120例疑似 AS 患者中,PCR-SBT 和 IMS-ELISA 法 HLA-B27檢測暘性率分彆為45.83%(55/120)和37.50%(45/120)。兩種方法檢測 HLA-B27差異有統計學意義(χ2=59.455,P =0.000)。PCR-SBT 法敏感度和特異度分彆為96.36%和96.92%;IMS-ELISA 法的敏感度和特異度分彆為69.09%和89.23%。兩者的 AUC 分彆為0.966和0.792。結論與 IMS-ELISA 法相比,在 AS 的臨床診斷中PCR-SBT 法檢測 HLA-B27的敏感度和特異度更高,方法更優。
목적:비교취합매련반응-직접측서법(polymerase chain reaction sequence-based typing,PCR-SBT)화자주매련면역법(immunomagnetic separation and enzyme-linked immunosorbent assay,IMS-ELISA)검측인류백세포항원 B27(human leukocyte antigen B27,HLA-B27)재강직성척주염(ankylosing spondylitis,AS)림상진단중적개치。방법응용 PCR-SBT법화 IMS-ELISA 법대120례의사 AS 환자외주혈진행 HLA-B27검측。이 SPSS17.0연건적배대χ2검험화수시자공작특정곡선하면적대량충방법검측 HLA-B27재 AS 진단중적개치진행평개。결과120례의사 AS 환자중,PCR-SBT 화 IMS-ELISA 법 HLA-B27검측양성솔분별위45.83%(55/120)화37.50%(45/120)。량충방법검측 HLA-B27차이유통계학의의(χ2=59.455,P =0.000)。PCR-SBT 법민감도화특이도분별위96.36%화96.92%;IMS-ELISA 법적민감도화특이도분별위69.09%화89.23%。량자적 AUC 분별위0.966화0.792。결론여 IMS-ELISA 법상비,재 AS 적림상진단중PCR-SBT 법검측 HLA-B27적민감도화특이도경고,방법경우。
Objective To compare PCR-SBT to IMS-ELISA in the HLA-B27 detection in the ankylosing spondylitis (AS)pa-tients.Methods Simultaneously,PCR-SBT and IMS-ELISA were used to detect the HLA-B27 expression in peripheral blood samples which were suspected patients with AS from 120 cases.Chisquare test of paired design and the area under curve of receiver operating characteristics of SPSS17.0 software were used to evaluate the value of PCR-SBT and IMS-ELISA in HLA-B27 detection of AS patients.Results Among 120 cases of suspected patients with AS,the positive rates of HLA-B27 detected by PCR-SBT and IMS-ELISA were 45.83%(55/120)and 37.50% (45/120),respectively.There was statistical difference between the two methods in the HLA-B27 detection (χ2 =59.455,P =0.000).The sensibility and spe-cificity of PCR-SBT were 96.36% and 96.92%,respectively.While the sensibility and the specificity of IMS-ELISA were 69.09% and 89.23%,respectively.Area under the curve of two methods were 0.966 and 0.792,respectively.Conclusion In comparison with IMS-ELISA,the sensibility and the specificity of PCR-SBT in HLA-B27 detection were higher in AS diag-nosis,that is to say,PCR-SBT is better in HLA-B27 detection and AS diagnosis.