CAJ | 학술논문

目的：了解儿童病毒性脑炎的病原体，建立快速、特异检测鼻病毒的方法，探讨病毒性脑炎与鼻病毒的相关性。方法收集2012年1月～12月全年期间收住在汕头大学医学院第二附属医院儿科重症监护室出现发热、惊厥症状的患儿脑脊液标本169份，应用巢式 RT-PCR 扩增脑脊液标本的鼻病毒核酸，对阳性结果进行核酸序列测定和同源性分析。结果巢式 RT-PCR 总共检测出39份鼻病毒阳性样本，总阳性检出率为23.1％；人类鼻病毒（HRV）感染的儿童中年龄在5岁以下的占87.6％；7～9月份 HRV 阳性检出率较高；序列分析显示39例 HRV 检测阳性标本中 HRV-A 型18例（46.1％，18／39），HRV-B 型7例（17.9％，7／39），HRV-C 型14例（35.9％，14／39）；28例病毒性脑炎有 8例检出 HRV，其中3例为HRV-C。结论建立的巢式 RT-PCR 可以检测到脑脊液标本中的鼻病毒片段，并且 HRV-A，B 和 C 三型均有检出，结合临床症状可认为 HRV 可能是病毒性脑炎的病原体之一。
목적：료해인동병독성뇌염적병원체，건립쾌속、특이검측비병독적방법，탐토병독성뇌염여비병독적상관성。방법수집2012년1월～12월전년기간수주재산두대학의학원제이부속의원인과중증감호실출현발열、량궐증상적환인뇌척액표본169빈，응용소식 RT-PCR 확증뇌척액표본적비병독핵산，대양성결과진행핵산서렬측정화동원성분석。결과소식 RT-PCR 총공검측출39빈비병독양성양본，총양성검출솔위23.1％；인류비병독（HRV）감염적인동중년령재5세이하적점87.6％；7～9월빈 HRV 양성검출솔교고；서렬분석현시39례 HRV 검측양성표본중 HRV-A 형18례（46.1％，18／39），HRV-B 형7례（17.9％，7／39），HRV-C 형14례（35.9％，14／39）；28례병독성뇌염유 8례검출 HRV，기중3례위HRV-C。결론건립적소식 RT-PCR 가이검측도뇌척액표본중적비병독편단，병차 HRV-A，B 화 C 삼형균유검출，결합림상증상가인위 HRV 가능시병독성뇌염적병원체지일。
Objective To understand the pathogen of viral encephalitis(VE)in children,and establish a rapid and specific method for detecting human rhinovirus(HRV),and investigate the correlation between HRV and viral encephalitis(VE). Methods 169 CSF specimens were collected from children with convulsions and fever,who were admitted to the pediatric intensive care unit (PICU)of Second Affiliated Hospital of Shantou University Medical College between January 2012 and December 2012.Nested RT-PCR was used to detected HRV in CSF specimens,and the positive PCR products were se-quenced,then analyzed and constructed the phylogenetic tree by software.Results 39 (23.1%)out of 169 samples were HRV positive.Among them,148(87.6%)children were below 5 years old.The detection rate of HRV increases from July to September,and reached its highest point in September.Sequence analyzed showed that the 39 HRV positive specimens inclu-ding 18(46.1%,18/39)positive for HRV-A,7(17.9%,7/39)positive for HRV-B,14(35.9%,14/39)positive for HRV-C. There were 8 out of 28 VE cases were detected in HRV,including 3(50%,3/6)positive for HRV-C.Conclusion HRV could be detected in CSF specimens by nested RT-PCR,including three types of HRV,combined with clinical symptoms con-sidered that HRV may be one of the VE pathogen.