CAJ | 학술논문

目的：研究广东省EV71病毒的流行病学特征和毒力的影响因素。方法：提取收集的样品或培养的病毒RNA、逆转录合成 cDNA，应用 PCR 技术扩增全长 EV71 VP1全长序列，应用 MEGA5．0进行序列比对、建设进化树，确定病毒的分型。用Logistic 回归分析对性别、年龄、病毒型别、VP1变异等因素对患者临床症状轻重的影响。结果：在2008－2010年期间，广东省流行的EV71病毒株为C4a 型，但该型病毒已经出现了分化，即C4a1-C4a4。患者的临床症状与性别、病毒的型别无相关性，在4岁之前，病毒变异A289T易致重症患者（P ＜0．05，OR ＝2．360，95％ CI 为1．163～4．659）。结论：在广东省 EV71流行株主要为 C4a1型，已经出现进化，且同时流行，需加强相关的监测；在流行期间需加强易感人群的防护。
목적：연구광동성EV71병독적류행병학특정화독력적영향인소。방법：제취수집적양품혹배양적병독RNA、역전록합성 cDNA，응용 PCR 기술확증전장 EV71 VP1전장서렬，응용 MEGA5．0진행서렬비대、건설진화수，학정병독적분형。용Logistic 회귀분석대성별、년령、병독형별、VP1변이등인소대환자림상증상경중적영향。결과：재2008－2010년기간，광동성류행적EV71병독주위C4a 형，단해형병독이경출현료분화，즉C4a1-C4a4。환자적림상증상여성별、병독적형별무상관성，재4세지전，병독변이A289T역치중증환자（P ＜0．05，OR ＝2．360，95％ CI 위1．163～4．659）。결론：재광동성 EV71류행주주요위 C4a1형，이경출현진화，차동시류행，수가강상관적감측；재류행기간수가강역감인군적방호。
Objective To study the factors influencing epidemiological characteristics and virulence of Enterovirus 71 (EV71) in Guangdong province from 2008 to 2010. Methods RNA was extracted from collected samples or cultured virus , then reversing transcription into cDNA. We amplified full-length EV71-VP1 using poly-merase chain reaction technology , then conducted sequence alignment and established phylogenetic tree with MEGA software (version 5.0) to confirm the genotype of EV71. The association between severity of clinical symp-toms and sex, age, viral genotype and VP1 variation was also analyzed using Logistic regression. Results The genotype of the predominant epidemical strain was C4a in Guangdong from 2008 to 2010. However , this subtype had already differentiated into 4 subgroups (C4a1- C4a4). There was no correlation between clinical syndrome and sex or viral genotype; the severity of symptoms was negatively correlated with age: before 4 years old, varia-tion A289T can easily lead to severe cases, increasing the risk of infection (P<0.05, OR = 2.360, 95%CI:1.163~ 4.659). Conclusion The main epidemical EV71 strain is C4a1 in Guangdong province. The emerging differen-tiation and simultaneous prevalence should merit attention to strengthen relevant surveillance; and the protection of the susceptible population should be reinforced during EV71 prevalence.