CAJ | 학술논문

目的：检测鼻咽癌组织中脾酪氨酸激酶（SYK）基因启动子区的甲基化状态，探讨 SYK 基因启动子区的甲基化与鼻咽癌发生的关系。方法收集2012年2月～2014年8月间在深圳市宝安区人民医院和北京大学深圳医院就诊的鼻咽癌患者52例，慢性鼻咽黏膜炎症患者26例，鼻咽癌和慢性鼻咽黏膜炎症患者均经病理学诊断证实。应用甲基化特异性PCR 技术检测鼻咽癌和慢性鼻咽黏膜炎症组织的 SYK 基因启动子区的甲基化状态，比较鼻咽癌和慢性鼻咽黏膜炎症组织的 SYK 基因启动子区的甲基化率。结果在52例鼻咽癌组织中有11例 SYK 基因启动子区存在甲基化，甲基化率为21.2％，而在26例慢性鼻咽黏膜炎症组织中未检测到 SYK 基因启动子区的甲基化。结论鼻咽癌组织中 SYK 基因启动子区存在较低程度的甲基化，这提示 SYK 基因启动子区出现的甲基化或许与鼻咽癌的发生相关。
목적：검측비인암조직중비락안산격매（SYK）기인계동자구적갑기화상태，탐토 SYK 기인계동자구적갑기화여비인암발생적관계。방법수집2012년2월～2014년8월간재심수시보안구인민의원화북경대학심수의원취진적비인암환자52례，만성비인점막염증환자26례，비인암화만성비인점막염증환자균경병이학진단증실。응용갑기화특이성PCR 기술검측비인암화만성비인점막염증조직적 SYK 기인계동자구적갑기화상태，비교비인암화만성비인점막염증조직적 SYK 기인계동자구적갑기화솔。결과재52례비인암조직중유11례 SYK 기인계동자구존재갑기화，갑기화솔위21.2％，이재26례만성비인점막염증조직중미검측도 SYK 기인계동자구적갑기화。결론비인암조직중 SYK 기인계동자구존재교저정도적갑기화，저제시 SYK 기인계동자구출현적갑기화혹허여비인암적발생상관。
Objective To detect methylation of spleen tyrosine kinase (SYK)gene promoter region in nasopharyngeal carcino-ma,and to explore relationship between carcinogenesis of nasopharyngeal carcinoma and methylation of SYK gene promoter region.Methods A total of 52 patients with nasopharyngeal carcinoma and 26 patients with chronic rhinitis from Baoan People’s Hospital of Shenzhen Hospital of Peking University were enrolled in this study between February 2012 and August 2014.All cases were diagnosed by pathological examination.Methylation specific-polymerase chain reaction assay was per-formed to detect methylation status of SYK gene promoter region,the rate of methylation was compared for patients with nasopharyngeal carcinoma and patients with chronic rhinitis.Results The methylation of promoter region of SYK gene was detected for 11 biopsy samples among 52 nasopharyngeal carcinoma patients,the methylation frequency was 21.2% in naso-pharyngeal carcinoma biopsy samples,while methylation was not found in 26 chronic rhinitis biopsy samples.Conclusion Low methylation of promoter region of SYK gene was found in nasopharyngeal carcinoma.It suggests that methylation of SYK gene promoter region may contribute to carcinogenesis of nasopharyngeal carcinoma.