CAJ | 학술논문

采用高频低场强脉冲电场系统对水中的大肠杆菌进行灭菌研究，探索反应器结构、电气参数及水质参数等对脉冲电场灭菌效率和能量效率的影响．圆筒式反应器体积为500 mL ，平均脉冲场强为3～12 kV／cm ，脉冲频率可以高达20 kHz ．最佳高压电极结构为6层均匀分布的直径为40 mm的网电极．脉冲电场的杀菌效率和能量效率随着脉宽和场强的增大而提高，水的电导率影响波形和单脉冲能量的变化，从而影响杀菌效果，当初始细菌密度降低时有利于彻底杀光．脉冲频率对杀菌无明显影响，但高频率能够大幅缩短处理时间．当脉冲电压为6 kV ，脉宽为30μs ，水的电导率为2．5μS／cm ，细胞初始密度为103～106 cf u／m L时，在消耗能量＜200 J／m L ，水温＜40℃的前提下，脉冲电场处理后细菌密度下降了1、2个对数．当细菌初始密度低于1250 cf u／m L时，细菌被全部杀光，实现了非热低温灭菌．
채용고빈저장강맥충전장계통대수중적대장간균진행멸균연구，탐색반응기결구、전기삼수급수질삼수등대맥충전장멸균효솔화능량효솔적영향．원통식반응기체적위500 mL ，평균맥충장강위3～12 kV／cm ，맥충빈솔가이고체20 kHz ．최가고압전겁결구위6층균균분포적직경위40 mm적망전겁．맥충전장적살균효솔화능량효솔수착맥관화장강적증대이제고，수적전도솔영향파형화단맥충능량적변화，종이영향살균효과，당초시세균밀도강저시유리우철저살광．맥충빈솔대살균무명현영향，단고빈솔능구대폭축단처리시간．당맥충전압위6 kV ，맥관위30μs ，수적전도솔위2．5μS／cm ，세포초시밀도위103～106 cf u／m L시，재소모능량＜200 J／m L ，수온＜40℃적전제하，맥충전장처리후세균밀도하강료1、2개대수．당세균초시밀도저우1250 cf u／m L시，세균피전부살광，실현료비열저온멸균．
Water disinfection of E .coli by high frequency pulsed low electric fields was investigated .The effects of constructional ,electrical and aqueous parameters on disinfection efficiency and energy efficiency were evaluated .Experiments were performed with a 500 mL cylindrical reactor .Average electric intensity of 3‐12 kV/cm was applied and pulsed frequency was up to 20 000 Hz .Optimized structure of high voltage electrode were six equally distributed mesh electrodes with diameter of 40 mm .Both the disinfection efficiency and energy efficiency were enhanced by increasing the electric strength and pulse duration .Water conductivity could influence the pulsed waveforms and single pulse energy ,which changed the disinfection results . Reducing the initial bacterial density made the process more effective and easily realized sterilization .Pulsed frequency did not show any effect on disinfection ,but high frequency could greatly reduce treatment time .When pulsed voltage ,pulsed width ,water conductivity and initial bacteria density were 6 kV ,30 μs ,2 .5 μS/cm and 103‐106 cfu/mL ,respectively ,1‐2 logarithmic reduction were obtained with energy consumption less than 200 J/mL and water temperature lower than 40 ℃ .If initial bacterial density was less than 1 250 cfu/mL ,all the cells could be sterilized ,which was a non‐thermal processing .