CAJ | 학술논문

目的研究葡萄糖调节蛋白78（GRP78）在胃癌、慢性萎缩性胃炎及浅表性胃炎组织中的表达及临床意义。方法 RT‐PCR法检测胃癌、慢性萎缩性胃炎和浅表性胃炎各25例新鲜组织中GRP78基因表达；免疫组化法检测胃癌、慢性萎缩性胃炎和浅表性胃炎各60例组织中GRP78蛋白表达。分析GRP78蛋白表达与临床病理参数间的相关性。结果胃癌组织中GRP78 mRNA的表达水平高于慢性萎缩性胃炎和浅表性胃炎（1.26±0.18 vs ．0.89±0.25和0.29±0.09）（ P＜0．01）。胃癌组织中GRP78蛋白的阳性表达率亦高于慢性萎缩性胃炎和浅表性胃炎（78.3％ vs ．46.6％和6.7％）（P＜0．01）。胃癌组织中GRP78蛋白表达与胃癌分化程度、分期、淋巴结转移等明显相关（P＜0．05或P＜0．01）。结论 GRP78在胃癌中呈高表达；检测GRP78的表达可能有助于预防及早期诊断胃癌。
목적연구포도당조절단백78（GRP78）재위암、만성위축성위염급천표성위염조직중적표체급림상의의。방법 RT‐PCR법검측위암、만성위축성위염화천표성위염각25례신선조직중GRP78기인표체；면역조화법검측위암、만성위축성위염화천표성위염각60례조직중GRP78단백표체。분석GRP78단백표체여림상병리삼수간적상관성。결과위암조직중GRP78 mRNA적표체수평고우만성위축성위염화천표성위염（1.26±0.18 vs ．0.89±0.25화0.29±0.09）（ P＜0．01）。위암조직중GRP78단백적양성표체솔역고우만성위축성위염화천표성위염（78.3％ vs ．46.6％화6.7％）（P＜0．01）。위암조직중GRP78단백표체여위암분화정도、분기、림파결전이등명현상관（P＜0．05혹P＜0．01）。결론 GRP78재위암중정고표체；검측GRP78적표체가능유조우예방급조기진단위암。
Objective To investigate the expression and clinical significance of glucose‐regulated protein 78(GRP78) in the patients with gastric carcinoma ,chronic atrophic gastritis and superficial gastritis .Methods The expressions of GRP78 mRNA in fresh tissues of gastric carcinoma (n= 25) ,chronic atrophic gastritis (n= 25) and superficial gastritis (n= 25) were detected by RT‐PCR .The expressions of GRP78 protein in gastric carcinoma tissues (n= 60) ,chronic atrophic gastritis tissues(n= 60) and superficial gastritis tissues (n= 60) were detected by immunohisto‐chemistry assay .The relationship between GRP78 expression and age ,gender ,differentiation , invasion ,disease stage and lymphoid node metastasis was analyzed .Results The expression level of GRP78 mRNA in gastric carcinoma tissues was significantly higher than that in the tissues of chronic atrophic gastritis and superficial gastritis(1.26 ± 0.18 vs .0.89 ± 0.25 and 0.29 ± 0.09)(P<0 .01) .So did the positive ratio of GRP78 protein expression(78.3% vs .46.6% and 6.7% )(P<0 .01) .GRP78 expression was positively correlated with tumor differentiation ,disease stage and lymphoid node metastasis(P<0 .05 or P<0 .01) .Conclusion GRP78 is highly expressed in the gastric carcinoma . Detection of GRP78 in tumor may be helpful for the prevention and early diagnosis of gastric carcinoma .