CAJ | 학술논문

目的探讨丰富环境对快速老化小鼠海马CA1区突触可塑性的影响.方法选用5月龄健康雄性SAMP8小鼠和SAMR1小鼠各24只,每种小鼠按随机数字法分为丰富环境组(EE)和标准环境组(SE)两组,各12只,在不同环境下饲养60d.通过Golgi染色检测海马CA1区顶树突树突棘的密度;免疫组化染色和图像分析系统检测海马CA1区突触素的表达;透射电镜技术、体视学方法和图像分析系统检测海马CA1区突触的数密度和面密度.结果 EE组SAMP8小鼠海马CA1区顶树突树突棘密度为(1.223±0.062)个/μm、突触素平均吸光度为(0.111±0.021)、突触的数密度为(3.742±0.052)个/μm3和面密度为(0.151±0.018)μm2/μm3;SE组SAMP8小鼠海马CA1区顶树突树突棘密度为(1.142±0.070)个/μm、突触素平均吸光度为(0.091±0.022)、突触的数密度为(3.626±0.049)个/μm3和面密度为(0.124±0.018)μm2/μm3,两组之间所有指标差异都具有统计学意义(均P＜0.01).EE组SAMR1小鼠与SE组SAMR1小鼠相比,所有指标之间差异也具有统计学意义(P＜0.05).结论丰富环境可调节海马CA1区神经元的突触可塑性,使树突棘的密度、突触素的表达、突触的数密度和面密度明显增加,这可能是其改善痴呆模型小鼠学习和记忆能力的作用机制.
목적 탐토봉부배경대쾌속노화소서해마CA1구돌촉가소성적영향.방법 선용5월령건강웅성SAMP8소서화SAMR1소서각24지,매충소서안수궤수자법분위봉부배경조(EE)화표준배경조(SE)량조,각12지,재불동배경하사양60d.통과Golgi염색검측해마CA1구정수돌수돌극적밀도;면역조화염색화도상분석계통검측해마CA1구돌촉소적표체;투사전경기술、체시학방법화도상분석계통검측해마CA1구돌촉적수밀도화면밀도.결과 EE조SAMP8소서해마CA1구정수돌수돌극밀도위(1.223±0.062)개/μm、돌촉소평균흡광도위(0.111±0.021)、돌촉적수밀도위(3.742±0.052)개/μm3화면밀도위(0.151±0.018)μm2/μm3;SE조SAMP8소서해마CA1구정수돌수돌극밀도위(1.142±0.070)개/μm、돌촉소평균흡광도위(0.091±0.022)、돌촉적수밀도위(3.626±0.049)개/μm3화면밀도위(0.124±0.018)μm2/μm3,량조지간소유지표차이도구유통계학의의(균P＜0.01).EE조SAMR1소서여SE조SAMR1소서상비,소유지표지간차이야구유통계학의의(P＜0.05).결론 봉부배경가조절해마CA1구신경원적돌촉가소성,사수돌극적밀도、돌촉소적표체、돌촉적수밀도화면밀도명현증가,저가능시기개선치태모형소서학습화기억능력적작용궤제.
Objective To explore the effects of enriched environment on synaptic plasticity in hippocampal CA1 region in senescence accelerated mouse.Methods Both twenty-four five-month-old healthy male SAMP8 and twenty-four five-month-old healthy male SAMR1 were randomly divided into enriched environment(EE) group and standard environment(SE) group(12 mice in each group).Mice in the 2 groups were subjected to the two different environments for 60 days.The apical dendritic thorns density in hippocampal CA1 region was measured by Golgi staining.The expression of synaptophysin in hippocampal CA1 was determined by immunohistochemical staining and image analysis system.The synaptic number density(Nv) and surface density(Sv) in hippocampal CA1 region were observed and analyzed by transmission electron microscope,stereology and image analysis system.Results For SAMP8 in EE group,the density of dendritic spines in hippocampal CA1 region was (1.223±0.062)/ μm,the average absorbance of synaptophysin was (0.111±0.021) and the parameters of Nv and Sv were (3.742± 0.052)/lμm3 and (0.151±0.018) μm2/μm3.For SAMP8 in SE group,the density of dendritic spines in hippocampal CA1 region was (1.142±0.070)/μm,the average absorbance of synaptophysin was (0.091 ±0.022) and the parameters of Nv and Sv were (3.626±0.049)/μm3 and (0.124±0.018)μm2/μm3.For all indicators,there was a significant difference between two groups (P＜0.01).There was statistically significant difference in all indicators between SAMR1 in EE group and SAMR1 in SE group(P＜0.05).Conclusion Enriched environment can adjust synaptic plasticity in hippocampal CA1 neurons,significantly increase the density of dendritic spines,the expression of synaptophysin,synaptic number density and surface density,which may be the mechanism of enriched environment improving the learning and memory ability in mice with dementia.