中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2015年
2期
101-104
,共4页
生命早期%高脂饮食%抑郁样行为%学习记忆%应激
生命早期%高脂飲食%抑鬱樣行為%學習記憶%應激
생명조기%고지음식%억욱양행위%학습기억%응격
Early life%High-fat diet%Depressive-like behavior%Learning and memory%Stress
目的 观察母鼠高脂饮食对子代成年后行为及学习记忆能力的影响,并初步探讨可能的机制.方法 孕期及哺乳期SD母鼠喂食高脂(high fat diet,HF)或标准饲料(normal diet,ND),子代雄性大鼠断奶后喂食标准饲料至120 d.随后母鼠喂食HF或ND的子代大鼠接受14 d正常饲养或慢性不可预见性应激(chronic unpredictable mild stress,CUMS),分为ND组,ND+ CUMS组,HF组以及HF+CUMS组(各9只);采用旷场实验、糖水偏好实验与强迫游泳实验评价各组抑郁样行为,采用Morris水迷宫评价学习记忆能力.另外两组正常饲养的ND及HF组(各8只)行心导管置管,基础及应激状态(束缚,1h)下采集血液,检测促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)及皮质酮(corticosterone,CORT)水平.结果 (1) HF组较ND组大鼠糖水偏好度降低(P＜0.05);与ND组比较,ND+CUMS组出现明显抑郁样行为.HF+CUMS组旷场实验的垂直运动得分、糖水偏好度[分别为(11.36±8.25)分,(0.63±0.04)]低于ND+CUMS组[分别为(17.12±7.54)分,(0.73±0.05)],而HF+CUMS组游泳不动时间[(33.16±6.35)s]高于ND+CUMS组[(25.74±7.31)s],均差异有统计学意义(P＜0.05).(2) Morris水迷宫实验中,HF组经过平台次数少于ND组(P＜0.05);与ND+CUMS组比较,HF+CUMS组目标象限时间、有效区域停留时间以及经过平台次数均显著减少(P＜0.05).(3)HF与ND两组大鼠血ACTH及CORT基础水平无明显差异(P＞0.05);1 h束缚中,激素水平均明显上升,但两组之间差异无统计学意义(P＞0.05).束缚结束3h内多个时间点,HF组ACTH及CORT水平均高于ND组(P＜0.05).结论 孕期及哺乳期(即子代生命发育早期)母体高脂饮食增加成年子代大鼠正常情况下抑郁样行为易感性及应激状态下的严重程度,并降低其学习记忆能力,这可能与应激状态下下丘脑-垂体-肾上腺轴活性改变相关.
目的 觀察母鼠高脂飲食對子代成年後行為及學習記憶能力的影響,併初步探討可能的機製.方法 孕期及哺乳期SD母鼠餵食高脂(high fat diet,HF)或標準飼料(normal diet,ND),子代雄性大鼠斷奶後餵食標準飼料至120 d.隨後母鼠餵食HF或ND的子代大鼠接受14 d正常飼養或慢性不可預見性應激(chronic unpredictable mild stress,CUMS),分為ND組,ND+ CUMS組,HF組以及HF+CUMS組(各9隻);採用曠場實驗、糖水偏好實驗與彊迫遊泳實驗評價各組抑鬱樣行為,採用Morris水迷宮評價學習記憶能力.另外兩組正常飼養的ND及HF組(各8隻)行心導管置管,基礎及應激狀態(束縳,1h)下採集血液,檢測促腎上腺皮質激素(adrenocorticotropic hormone,ACTH)及皮質酮(corticosterone,CORT)水平.結果 (1) HF組較ND組大鼠糖水偏好度降低(P＜0.05);與ND組比較,ND+CUMS組齣現明顯抑鬱樣行為.HF+CUMS組曠場實驗的垂直運動得分、糖水偏好度[分彆為(11.36±8.25)分,(0.63±0.04)]低于ND+CUMS組[分彆為(17.12±7.54)分,(0.73±0.05)],而HF+CUMS組遊泳不動時間[(33.16±6.35)s]高于ND+CUMS組[(25.74±7.31)s],均差異有統計學意義(P＜0.05).(2) Morris水迷宮實驗中,HF組經過平檯次數少于ND組(P＜0.05);與ND+CUMS組比較,HF+CUMS組目標象限時間、有效區域停留時間以及經過平檯次數均顯著減少(P＜0.05).(3)HF與ND兩組大鼠血ACTH及CORT基礎水平無明顯差異(P＞0.05);1 h束縳中,激素水平均明顯上升,但兩組之間差異無統計學意義(P＞0.05).束縳結束3h內多箇時間點,HF組ACTH及CORT水平均高于ND組(P＜0.05).結論 孕期及哺乳期(即子代生命髮育早期)母體高脂飲食增加成年子代大鼠正常情況下抑鬱樣行為易感性及應激狀態下的嚴重程度,併降低其學習記憶能力,這可能與應激狀態下下丘腦-垂體-腎上腺軸活性改變相關.
목적 관찰모서고지음식대자대성년후행위급학습기억능력적영향,병초보탐토가능적궤제.방법 잉기급포유기SD모서위식고지(high fat diet,HF)혹표준사료(normal diet,ND),자대웅성대서단내후위식표준사료지120 d.수후모서위식HF혹ND적자대대서접수14 d정상사양혹만성불가예견성응격(chronic unpredictable mild stress,CUMS),분위ND조,ND+ CUMS조,HF조이급HF+CUMS조(각9지);채용광장실험、당수편호실험여강박유영실험평개각조억욱양행위,채용Morris수미궁평개학습기억능력.령외량조정상사양적ND급HF조(각8지)행심도관치관,기출급응격상태(속박,1h)하채집혈액,검측촉신상선피질격소(adrenocorticotropic hormone,ACTH)급피질동(corticosterone,CORT)수평.결과 (1) HF조교ND조대서당수편호도강저(P＜0.05);여ND조비교,ND+CUMS조출현명현억욱양행위.HF+CUMS조광장실험적수직운동득분、당수편호도[분별위(11.36±8.25)분,(0.63±0.04)]저우ND+CUMS조[분별위(17.12±7.54)분,(0.73±0.05)],이HF+CUMS조유영불동시간[(33.16±6.35)s]고우ND+CUMS조[(25.74±7.31)s],균차이유통계학의의(P＜0.05).(2) Morris수미궁실험중,HF조경과평태차수소우ND조(P＜0.05);여ND+CUMS조비교,HF+CUMS조목표상한시간、유효구역정류시간이급경과평태차수균현저감소(P＜0.05).(3)HF여ND량조대서혈ACTH급CORT기출수평무명현차이(P＞0.05);1 h속박중,격소수평균명현상승,단량조지간차이무통계학의의(P＞0.05).속박결속3h내다개시간점,HF조ACTH급CORT수평균고우ND조(P＜0.05).결론 잉기급포유기(즉자대생명발육조기)모체고지음식증가성년자대대서정상정황하억욱양행위역감성급응격상태하적엄중정도,병강저기학습기억능력,저가능여응격상태하하구뇌-수체-신상선축활성개변상관.
Objective To investigate the effects of high-fat feeding during pregnancy and lactation on the behaviors and learning memory in adult male offspring,as well as explore its possible mechanisms.Methods Female SD rats were exposed to either high fat diet (HF) or normal diet (ND) during pregnancy and lactation period.From weaning,all male offspring were fed with ND until 120-day.The offspring whose mothers were fed with HF or ND received 14 d chronic unpredictable mild stress (CUMS) or normal circumstance,and being divided into ND group,ND+CUMS group,HF group and HF+CUMS group(9 rats in each group).The Open-field test,sucrose preference test and forced swimming test were used to evaluate the depressive-like behaviors,and Morris water maze test was employed to assess the learning and memory ability.Moreover,blood samples were taken via chronically implanted cardiac catheters for measurement of adrenocorticotropic hormone (ACTH) and corticosterone (CORT) levels in another set of DN group and HF group with normal circumstance during baseline and restraint stress (1 h).Results (1) Compared with the ND group,the sucrose preference in the HF group were decreased (P＜0.05).Compared with ND group,ND +CUMS group showed more serious depressive-like behavior.Vertical score in open-field test (11.36±8.25) and sucrose preference(0.63±0.04) of HF+CUMS group,were lower than those of the ND+CUMS group ((17.12±7.54),(0.73±0.05),respectively).The immobility time in forced swimming test of HF+CUMS group ((33.16±6.35)s) were longer than that of ND+CUMS group ((25.74±7.31) s).(2) In Morris water maze test,the crossing platform times of the HF group was less than those of the HF group.Compared with ND+CUMS group,the target quadrant time,effective area residence time and crossing platform times of HF+CUMS group were decreased significantly (P＜0.05).(3)There was no differences in basal ACTH and CORT between ND group and HF group (P＞0.05).Both ND group and HF group exhibited significantly elevated levels of plasma ACHT and CORT during restraint,but without significant difference between these two groups (P＞ 0.05).Within 3 h after restraint,the HF group showed significant increase of ACTH and CORT compared with ND group(P＜0.05).Conclusion As one kind of distress in early life,high-fat geeding through pregnancy and lactation increase the susceptibility and severity of depressive-like behaviors in adult offspring,as well as reducing the learning and memory ability,and the activity of hypothalamic-pituitary-adrenal axis activity during stress may contribute to the changes.
