中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2015年
2期
73-77
,共5页
于立新%曾明星%叶桂荣%罗敏%肖露露
于立新%曾明星%葉桂榮%囉敏%肖露露
우립신%증명성%협계영%라민%초로로
IgA肾病%慢性肾功能衰竭%人类白细胞抗原%多态性%高分辨%聚合酶链反应-直接测序分型技术
IgA腎病%慢性腎功能衰竭%人類白細胞抗原%多態性%高分辨%聚閤酶鏈反應-直接測序分型技術
IgA신병%만성신공능쇠갈%인류백세포항원%다태성%고분변%취합매련반응-직접측서분형기술
Immunoglobulin a nephropathy%Chronic renal failure%Human Leukocyte Antigens%Polymorphism%High-resolution%Poymerase chain reaction-sequence-based typing
目的 探讨HLA-A、B、DRB1高分辨等位基因表达与因IgA肾病(IgAN)最终导致慢性肾功能衰竭(CRF)的相关性.方法 采用聚合酶链反应直接测序分型技术(PCR-SBT)对来自于广东省3所三级甲等医院共191例因IgAN所致CRF患者(研究组)和503例健康献血者(对照组)进行HLA-A、B、DRB1高分辨等位基因分型,比较两组等位基因的频率,分析因IgAN所致CRF与HLA基因多态性的关系.结果 (1)研究组中共表达出HLA等位基因A位点25个,B位点48个,DRB1位点32个.(2)与对照组比较,研究组HLA-A* 2901 (Pc=0.033,OR=10.738,95％ CI为1.193～96.691),HLA DRB1*1106 (Pc=0.0001,OR=0.969,95％CI为0.944～0.994),HLA-DRB1*1202(Pc=0.002,OR=1.859,95％ CI为1.259～2.745),HLA-DRB1*1401 (Pc=0.021,OR=0.984,95％ CI为0.967～0.998),HLA-DRB1* 1602(Pc=0.015,OR=1.915,95％ CI为1.157～3.17)基因频率较对照组显著升高,差异均有统计学意义.结论 HLA-A* 2901及HLA-DRB1*1106、*1202、* 1401和*1602高分辨等位基因与IgAN所致CRF呈易感关联,由此推测HLA基因多态性表达与IgAN所致CRF的发生可能有密切的遗传免疫关联性.
目的 探討HLA-A、B、DRB1高分辨等位基因錶達與因IgA腎病(IgAN)最終導緻慢性腎功能衰竭(CRF)的相關性.方法 採用聚閤酶鏈反應直接測序分型技術(PCR-SBT)對來自于廣東省3所三級甲等醫院共191例因IgAN所緻CRF患者(研究組)和503例健康獻血者(對照組)進行HLA-A、B、DRB1高分辨等位基因分型,比較兩組等位基因的頻率,分析因IgAN所緻CRF與HLA基因多態性的關繫.結果 (1)研究組中共錶達齣HLA等位基因A位點25箇,B位點48箇,DRB1位點32箇.(2)與對照組比較,研究組HLA-A* 2901 (Pc=0.033,OR=10.738,95％ CI為1.193～96.691),HLA DRB1*1106 (Pc=0.0001,OR=0.969,95％CI為0.944～0.994),HLA-DRB1*1202(Pc=0.002,OR=1.859,95％ CI為1.259～2.745),HLA-DRB1*1401 (Pc=0.021,OR=0.984,95％ CI為0.967～0.998),HLA-DRB1* 1602(Pc=0.015,OR=1.915,95％ CI為1.157～3.17)基因頻率較對照組顯著升高,差異均有統計學意義.結論 HLA-A* 2901及HLA-DRB1*1106、*1202、* 1401和*1602高分辨等位基因與IgAN所緻CRF呈易感關聯,由此推測HLA基因多態性錶達與IgAN所緻CRF的髮生可能有密切的遺傳免疫關聯性.
목적 탐토HLA-A、B、DRB1고분변등위기인표체여인IgA신병(IgAN)최종도치만성신공능쇠갈(CRF)적상관성.방법 채용취합매련반응직접측서분형기술(PCR-SBT)대래자우광동성3소삼급갑등의원공191례인IgAN소치CRF환자(연구조)화503례건강헌혈자(대조조)진행HLA-A、B、DRB1고분변등위기인분형,비교량조등위기인적빈솔,분석인IgAN소치CRF여HLA기인다태성적관계.결과 (1)연구조중공표체출HLA등위기인A위점25개,B위점48개,DRB1위점32개.(2)여대조조비교,연구조HLA-A* 2901 (Pc=0.033,OR=10.738,95％ CI위1.193～96.691),HLA DRB1*1106 (Pc=0.0001,OR=0.969,95％CI위0.944～0.994),HLA-DRB1*1202(Pc=0.002,OR=1.859,95％ CI위1.259～2.745),HLA-DRB1*1401 (Pc=0.021,OR=0.984,95％ CI위0.967～0.998),HLA-DRB1* 1602(Pc=0.015,OR=1.915,95％ CI위1.157～3.17)기인빈솔교대조조현저승고,차이균유통계학의의.결론 HLA-A* 2901급HLA-DRB1*1106、*1202、* 1401화*1602고분변등위기인여IgAN소치CRF정역감관련,유차추측HLA기인다태성표체여IgAN소치CRF적발생가능유밀절적유전면역관련성.
Objective To investigate the correlation between human leukocyte antigens-A,-B,-DRB1 (HLA-A,-B,-DRB1) high resolution alleles and chronic renal failure (CRF) caused by immunoglobulin-a nephropathy (IgAN).Method The polymerase chain reaction-sequence-based typing (PCR-SBT) method was used to investigate the genotypes of HLA-A,-B and-DRB1 high-resolution alleles in 191 cases of CRF caused by IgAN (experimental group) and 503 healthy blood donors (control group).The alleles frequencies between two groups were compared and the association between CRF caused by IgAN and the polyrnorphism of HLA was analyzed.Result (1) There were 25 alleles at A locus,48 alleles at B locus and 32 alleles at DRB1 locus in experimental group.(2) The genetic frequency of HLAA * 2901 [Pc =0.033,OR =10.738,95％ CI (1.193,96.691)],HLA DRB1 * 1106 [Pc =0.0001,OR =0.969,95％ CI (0.944,0.994)],HLA-DRB1 * 1202[Pc =0.002,OR =1.859,95％ CI (1.259,2.745)],HLA-DRB1 * 1401 [Pc =0.021,OR =0.984,95％ CI (0.967,0.998)],HLA-DRB1 * 1602[Pc=0.015,OR=1.915,95％ CI (1.157,3.17)] in experimental group was higher than in control group (P＜0.05).Conclusion There is susceptibility association of HLA-A * 2901,HLA-DRB1 * 1106,HLA-DRE * 1202,HLA-DRB1 * 1401,HLA-DRB1 * 1602 with CRF caused by IgAN.It is concluded that there is a close genetic and immunological correlation between HLA alleles and the pathogenesis of CRF caused by IgAN.
