CAJ | 학술논문

目的：研究强直性脊柱炎（AS）不同活动期辅助性 T 细胞1（Th1）、辅助性 T 细胞2（Th2）和调节性 T细胞（Treg）量的改变及临床意义。方法：采用细胞内因子染色技术（ICS）及流式细胞术（FCM）检测78例 AS 患者（其中高活动性 AS 34例、低活动性 AS 44例）和30例健康体检者（正常对照组）外周血中 Th1细胞、Th2细胞和 Treg 细胞数量，分析其与 AS 活动性的相关性。结果：在高活动性 AS 组、低活动性 AS 组和 AS 组外周血 Th1细胞的量均明显高于正常对照组（P ＜0．01），低活动性 AS 组外周血 Th1细胞的量明显低于高活动性 AS 组（P ＜0．01）；高活动性 AS 组和 AS 组外周血 Th2细胞的量均明显低于正常对照组和低活动性 AS 组（P ＜0．05）；AS 患者外周血 Treg 细胞，AS 疾病组与正常对照组差异无统计学意义（P ＞0．05），AS 患者低活动性组外周血 Treg 细胞与正常对照组差异无统计学意义（P ＞0．05），但高活动性 AS 组外周血 Treg 细胞与对照组比较明显减低，差异有统计学意义（P ＜0．01），AS 患者高活动性组外周血 Treg 细胞与 AS 患者低活动性组比较明显减低，差异有统计学意义（P ＜0．01）；相关性分析显示，AS 患者外周血 Th1细胞比例与 Bath 强直性脊柱炎病情活动指数（BAS-DI）呈正相关，Th2细胞和 Treg 细胞与 BASDI 均呈负相关。结论：AS 患者外周血 Th1、Th2、Treg 细胞数量的改变，与 AS 的发生、发展有明显的相关性，检测 AS 患者外周血 Th1、Th2、Treg 细胞的数量有助于 AS 病情的判断。
목적：연구강직성척주염（AS）불동활동기보조성 T 세포1（Th1）、보조성 T 세포2（Th2）화조절성 T세포（Treg）량적개변급림상의의。방법：채용세포내인자염색기술（ICS）급류식세포술（FCM）검측78례 AS 환자（기중고활동성 AS 34례、저활동성 AS 44례）화30례건강체검자（정상대조조）외주혈중 Th1세포、Th2세포화 Treg 세포수량，분석기여 AS 활동성적상관성。결과：재고활동성 AS 조、저활동성 AS 조화 AS 조외주혈 Th1세포적량균명현고우정상대조조（P ＜0．01），저활동성 AS 조외주혈 Th1세포적량명현저우고활동성 AS 조（P ＜0．01）；고활동성 AS 조화 AS 조외주혈 Th2세포적량균명현저우정상대조조화저활동성 AS 조（P ＜0．05）；AS 환자외주혈 Treg 세포，AS 질병조여정상대조조차이무통계학의의（P ＞0．05），AS 환자저활동성조외주혈 Treg 세포여정상대조조차이무통계학의의（P ＞0．05），단고활동성 AS 조외주혈 Treg 세포여대조조비교명현감저，차이유통계학의의（P ＜0．01），AS 환자고활동성조외주혈 Treg 세포여 AS 환자저활동성조비교명현감저，차이유통계학의의（P ＜0．01）；상관성분석현시，AS 환자외주혈 Th1세포비례여 Bath 강직성척주염병정활동지수（BAS-DI）정정상관，Th2세포화 Treg 세포여 BASDI 균정부상관。결론：AS 환자외주혈 Th1、Th2、Treg 세포수량적개변，여 AS 적발생、발전유명현적상관성，검측 AS 환자외주혈 Th1、Th2、Treg 세포적수량유조우 AS 병정적판단。
Objective:To investigate the changes of T helper cells 1 (Th1),T helper cells 2 (Th2) and regulatory T cells (Treg)in the peripheral blood of ankylosing spondylitis (AS)patients in the different active stages of development of AS and their clinical significance.Methods:Seventy-eight cases of AS patients were selected as AS group and 30 cases as healthy group (normal control group), and AS group was divided into low activity AS group (44 cases)and high activity AS group (34 ca-ses).The flow cytometry(FCM)and intracellular cytokine staining technique(ICS)were adopted to determine the number of Th1,Th2 and Treg cells in peripheral blood of all groups and analyzed the correlation between these cell number and AS activity.Results:The number of Th1 cells in high-ac-tivity AS group,low-activity AS group and AS group was signifcantly higher than that of normal control group (P <0.01 ),while the number of Th1 cells in low-activity AS group were significantly lower than that in high-activity group(P <0.01).The number of Th2 cells in high-activity AS group and AS group were significantly lower than that in low-activity group and control group (P <0.01).There was no statistical difference in the number of Treg cells between low-activity AS group,AS group and nor-mal control group (P >0.05).The number of Treg cells in high-activity AS group was significantly <br> lower than that in low-activity AS group and normal control group (P <0.01 ).Correlation analysis showed the percentage of Th1 cells was positively correlated to bath ankylosing spondylitis disease ac-tivity index (BASDI)while the percentage of Th2 and Treg cells was negatively correlated to BASDI. Conclusions:The percentage changes of Th1 cells,Treg cells in the peripheral blood of AS patients are significantly related to occurrence and development of AS.The detection of Th1 cells,Th2 cells and Treg cells in the peripheral blood can contribute to judgement of AS disease.