CAJ | 학술논문

目的：分析 MSCT 征象与胃癌组织中 VEGF-C 基因表达及淋巴管密度间的关系。方法收集58例胃癌肿瘤组织及对应正常组织放置液氮及低温冰箱中冷冻,后行 RT-PCR 检测组织中 VEGF-C mRNA 表达,行酶组织化学染色检测组织淋巴管密度。并回顾性分析该组患者术前的 MSCT 平扫及动态增强扫描图像。结果肿瘤组织标本中 VEGF-C mRNA 阳性率74.1%及淋巴结密度6.82±3.43,均高于正常组织中 VEGF-C mRNA 阳性率50.0%及淋巴管密度2.15±1.56(P <0.05)；肿瘤组织中VEGF-C 表达与淋巴管密度呈正相关(P <0.05)；在 MSCT 诊断的弥漫型与肠型组、淋巴结转移组与未转移组中 VEGF-C mRNA阳性表达率分别为87.1%和59.3%、87.8%和41.2%,对应组织淋巴管密度分别为8.04±4.58和4.08±2.44、8.50±4.70和3.64±1.41,各组间差异均具有统计学意义(P <0.05)。结论胃癌中 VEGF-C 基因高表达及较高淋巴管密度与 CT 征象上淋巴结转移及 Lauren 分型相关。VEGF-C 通过促进淋巴管的形成影响肿瘤的淋巴道转移。
목적：분석 MSCT 정상여위암조직중 VEGF-C 기인표체급림파관밀도간적관계。방법수집58례위암종류조직급대응정상조직방치액담급저온빙상중냉동,후행 RT-PCR 검측조직중 VEGF-C mRNA 표체,행매조직화학염색검측조직림파관밀도。병회고성분석해조환자술전적 MSCT 평소급동태증강소묘도상。결과종류조직표본중 VEGF-C mRNA 양성솔74.1%급림파결밀도6.82±3.43,균고우정상조직중 VEGF-C mRNA 양성솔50.0%급림파관밀도2.15±1.56(P <0.05)；종류조직중VEGF-C 표체여림파관밀도정정상관(P <0.05)；재 MSCT 진단적미만형여장형조、림파결전이조여미전이조중 VEGF-C mRNA양성표체솔분별위87.1%화59.3%、87.8%화41.2%,대응조직림파관밀도분별위8.04±4.58화4.08±2.44、8.50±4.70화3.64±1.41,각조간차이균구유통계학의의(P <0.05)。결론위암중 VEGF-C 기인고표체급교고림파관밀도여 CT 정상상림파결전이급 Lauren 분형상관。VEGF-C 통과촉진림파관적형성영향종류적림파도전이。
Objective To evaluated the correlations between MSCT features and expression of VEGF-C,lymphatic vessel density (LVD)in gastric carcinoma.Methods Both plain MSCT and triphasic dynamic contrast-enhanced scan were performed in 58 patients with gastric carcinoma.All patients underwent total/subtotal gastrectomy after MSCT scanning.All specimens were collected into liquid nitrogen or deep freeze refrigerator.Detection procedure for VEGF-C mRNA was performed using RT-PCR,and the LVD was detected with 5’-nucleotidase (5’-Nase)histochemistry.Results The VEGF-C positive rate and the LVD in tumor tissue were high-er than those in normal tissue (P < 0.05 ).In the tumors between diffused and intestinal groups and between non-metastasis and lymph node metastasis groups,the VEGF-C positive rate was 87.1% and 59.3%,87.8% and 41.2%,and the LVD was 8.04±4.58 and 4.08±2.44,8.50±4.70 and 3.64 ± 1.41,respectively,indicating statistically significant differences (P <0.05 ).Conclusion Over-expression of VEGF-C and higher LVD are closely correlated with the lymph node metastasis and Lauren types of MSCT fea-tures of gastric carcinoma.VEGF-C can promote the lymphangiogenesis in carcinoma and further lymph metastasis.