CAJ | 학술논문

目的：在细胞水平探索miR－21在调控TGF－β1诱导的大鼠骨髓间充质干细胞向肌成纤维细胞分化中的作用。方法：全髓培养法培养原代大鼠骨髓间充质干细胞，培养至第三代时用TGF－β1分组诱导培养，检测TGF－β1对大鼠BMSCs促纤维化的作用及该过程中不同浓度梯度TGF－β1诱导以及不同时间段miR－21的表达变化；通过转染miR－21 mimics（高表达）不同时间点检测其对α－SMA表达的影响。结果：TGF－β1能促进大鼠骨髓间充质干细胞向成纤维，肌成纤维细胞分化；大鼠骨髓间充质干细胞向成纤维，肌成纤维细胞分化后miR－21表达上调；上调miR－21能促进大鼠BMSCs的纤维化作用。结论：miR－21 mimics能够促进大鼠BMSCs的纤维化作用。
목적：재세포수평탐색miR－21재조공TGF－β1유도적대서골수간충질간세포향기성섬유세포분화중적작용。방법：전수배양법배양원대대서골수간충질간세포，배양지제삼대시용TGF－β1분조유도배양，검측TGF－β1대대서BMSCs촉섬유화적작용급해과정중불동농도제도TGF－β1유도이급불동시간단miR－21적표체변화；통과전염miR－21 mimics（고표체）불동시간점검측기대α－SMA표체적영향。결과：TGF－β1능촉진대서골수간충질간세포향성섬유，기성섬유세포분화；대서골수간충질간세포향성섬유，기성섬유세포분화후miR－21표체상조；상조miR－21능촉진대서BMSCs적섬유화작용。결론：miR－21 mimics능구촉진대서BMSCs적섬유화작용。
Objective:To investigate the role of miR-21 in the jaw of fibrosis in vitro. Method:Whole bone marrow adherence on plastic was used to isolation and culture rat mesenchymal stem cells. When passage 3, the cells were treated with TGF-β1 for different periods of time and concentration. The target gene expression of miR-21 was detected by real-time PCR;Over-expression of miR-21 by niR-21 mimics,to study whether up-regulated miR-21 enhances myfibroblast differentiation of rat mesenchymal stem cells by immunohistochemistry. Result:Real-time PCR demonstrated that TGF-β1 induced miR-21 expression in a time-dependent and dosage-dependent manner,peaking at 48h with an optimal dose at 5 ng/mL. The protein expression of α-SMA was up-regulated. Conclusion:miR-21 could promote myfibroblast differentia-tion of BMSCs in vitro. Inhibition of miR-21 may be a therapeutic approach to suppress renal fibrosis.