CAJ | 학술논문

目的：探讨吸烟与口腔黏膜上皮细胞NOD1信号通路关键分子表达水平的关系。方法：采用商业化可购的直接烟暴露装置，构建体外Leuk－1细胞吸烟模型，将其分为吸烟组（分别吸入香烟烟雾5、10、20、40、60 min）和对照组（吸入含5％CO2空气10 min），应用间接免疫荧光法检测体外吸烟对口腔黏膜上皮细胞NOD1通路内关键蛋白的表达。结果：随着体外吸烟时间的延长，NOD1蛋白表达水平逐渐下降，Caspase12和RIP2蛋白表达水平逐渐上升。NF－κB的蛋白表达水平逐渐上升之后明显下降。结论：吸烟可使口腔黏膜细胞NOD1信号通路相关蛋白表达发生改变，进而影响口腔黏膜固有免疫。
목적：탐토흡연여구강점막상피세포NOD1신호통로관건분자표체수평적관계。방법：채용상업화가구적직접연폭로장치，구건체외Leuk－1세포흡연모형，장기분위흡연조（분별흡입향연연무5、10、20、40、60 min）화대조조（흡입함5％CO2공기10 min），응용간접면역형광법검측체외흡연대구강점막상피세포NOD1통로내관건단백적표체。결과：수착체외흡연시간적연장，NOD1단백표체수평축점하강，Caspase12화RIP2단백표체수평축점상승。NF－κB적단백표체수평축점상승지후명현하강。결론：흡연가사구강점막세포NOD1신호통로상관단백표체발생개변，진이영향구강점막고유면역。
Objective:To evaluate the effect of Cigarette Smoke on Key Molecules of NOD1 Signaling in human oral mucosal epithelial cells. Method:The cells were stimulated using a smoke chamber with cigarette smoke for different times (5、10、20、40、60 min) in certain experiments. The control group cells were cultured with 5 % CO2 for 10 min. The crucial protein expression in NOD1 Signaling was measured by Immunofluorescence assays. Result: Cigarette Smoke down-regu-lated NOD1 protein levels and up-regulated Caspase 12 and RIP2 protein in a time-dependent manner. The NF-κB protein level reached the peak level after stimulated for 10 mins. Conclusion:Cigarette smoke could play a inhibitory effects on NOD1 Signaling even innate immunity in human oral mucosal epithelial cells.