CAJ | 학술논문

目的：制作亚急性肝内胆汁淤积动物模型，探索其发生的生化和病理变化过程。方法幼年SD大鼠40只，分为α-萘异硫氰酸酯（ ANIT）模型组和对照组，分别给予ANIT及芝麻油灌胃，1次／周，共4次，每次灌胃后48 h处死一批大鼠，取血标本送检肝功能生化指标，留取肝脏标本观察肝脏以及胆管病理改变。结果 ANIT灌胃后48 h，血转氨酶、胆红素明显上升，肝脏出现结构坏死，炎症细胞浸润、增生，胆管增生，第9天、第16天上述病变逐渐加重，第23天肝脏生化指标逐渐恢复，肝脏结构好转，胆管增生和纤维化仍明显。结论成功制作幼鼠亚急性胆汁淤积的动物模型，ANIT每周一次灌胃后2～3周内胆汁淤积的生化和病理改变明显，第4周逐渐恢复，但胆管增生和肝纤维化仍然明显。
목적：제작아급성간내담즙어적동물모형，탐색기발생적생화화병리변화과정。방법유년SD대서40지，분위α-내이류청산지（ ANIT）모형조화대조조，분별급여ANIT급지마유관위，1차／주，공4차，매차관위후48 h처사일비대서，취혈표본송검간공능생화지표，류취간장표본관찰간장이급담관병리개변。결과 ANIT관위후48 h，혈전안매、담홍소명현상승，간장출현결구배사，염증세포침윤、증생，담관증생，제9천、제16천상술병변축점가중，제23천간장생화지표축점회복，간장결구호전，담관증생화섬유화잉명현。결론성공제작유서아급성담즙어적적동물모형，ANIT매주일차관위후2～3주내담즙어적적생화화병리개변명현，제4주축점회복，단담관증생화간섬유화잉연명현。
Objective To manufacture the sub-acute intrahepatic cholestasis model with infantile rats and explore the biochemical and pathological changes .Methods Male SD infantile rats (150~180 g, n=40 ) were randomly di-vided into two groups .The ANIT ( alpha-naphthyl-isothiocyanate ) model group was lavaged with four weekly doses of ANIT (80 mg/kg), the control group was lavaged with same volume of placebo sesame oil .Eight rats were sacrificed af-ter 48 hour ANIT treatment every week .The blood samples were collected to assess AST , ALT, TBIL, DBIL, TBA, ALP and GGT, and the liver samples were made into paraffin sections and observed with microscope .Results Serum ALT, TBIL, DBIL, TBA, ALP and GGT were increased after 48 hour ANIT treatment, and maintained at the high level until the third week.In the fourth week, the serum ALT, TBIL, DBIL, ALP were declined quickly, however the serum TBA and GGT remained rising .Pathological changes were obvious after 48 h first ANIT treatment , characterizing with liver necrosis , inflammatory infiltration , bile duct hyperplasia , which appeared gradually increasing until the third week . In the fourth week , the liver necrosis and inflammatory infiltration recovered normal , but the bile duct hyperplasia and liver fibrosis remained aggravating .Conclusion The sub-acute intraheptic cholestasis model is manufactured successful-ly with the ANIT lavaging weekly .The cholestasis changes are the most obvious in the second and third week , but reco-vere in the fourth week except the bile duct hyperplasia and liver fibrosis .