光明中医
光明中醫
광명중의
GUANG MING JOURNAL TRADITIONAL CHINESE MEDICINE
2015年
4期
736-739
,共4页
膝骨关节炎%昆明小鼠%木瓜蛋白酶%L-半胱氨酸%动物模型
膝骨關節炎%昆明小鼠%木瓜蛋白酶%L-半胱氨痠%動物模型
슬골관절염%곤명소서%목과단백매%L-반광안산%동물모형
Knee osteoarthritis%Kunming mouce%Papain%L-cysteine%Animal model
目的：探讨关节腔内注射不同浓度木瓜蛋白酶建立小鼠膝骨关节炎模型的可行性。方法健康10周龄雄性昆明小鼠80只，体重（45±3） g，随机分为2％木瓜蛋白酶组、4％木瓜蛋白酶组、8％木瓜蛋白酶组和对照组。实验第1、3、5天分组行右膝关节腔内注射2％、4％、8％（ w／v）木瓜蛋白酶和0．3mol／LL－半胱氨酸的混合溶液0．25 mL／kg或等量生理盐水。记录小鼠一般情况及右膝关节宽度。所有小鼠首次注射1周后处死，取关节行常规HE染色及甲苯胺蓝染色，镜下观察并评分。结果造模组右膝关节出现肿胀、功能障碍；右膝关节软骨染色变浅、软骨层变薄、基质分解破坏等病理学改变，其严重程度与木瓜蛋白酶浓度呈正相关；对照组无上述改变。结论膝关节腔内注射2％、4％、8％（ w／v）木瓜蛋白酶和0．3mol／L L－半胱氨酸的混合溶液0．25 mL／kg可建立小鼠不同严重程度的膝骨关节炎，周期短、成功率高。
目的：探討關節腔內註射不同濃度木瓜蛋白酶建立小鼠膝骨關節炎模型的可行性。方法健康10週齡雄性昆明小鼠80隻，體重（45±3） g，隨機分為2％木瓜蛋白酶組、4％木瓜蛋白酶組、8％木瓜蛋白酶組和對照組。實驗第1、3、5天分組行右膝關節腔內註射2％、4％、8％（ w／v）木瓜蛋白酶和0．3mol／LL－半胱氨痠的混閤溶液0．25 mL／kg或等量生理鹽水。記錄小鼠一般情況及右膝關節寬度。所有小鼠首次註射1週後處死，取關節行常規HE染色及甲苯胺藍染色，鏡下觀察併評分。結果造模組右膝關節齣現腫脹、功能障礙；右膝關節軟骨染色變淺、軟骨層變薄、基質分解破壞等病理學改變，其嚴重程度與木瓜蛋白酶濃度呈正相關；對照組無上述改變。結論膝關節腔內註射2％、4％、8％（ w／v）木瓜蛋白酶和0．3mol／L L－半胱氨痠的混閤溶液0．25 mL／kg可建立小鼠不同嚴重程度的膝骨關節炎，週期短、成功率高。
목적：탐토관절강내주사불동농도목과단백매건립소서슬골관절염모형적가행성。방법건강10주령웅성곤명소서80지，체중（45±3） g，수궤분위2％목과단백매조、4％목과단백매조、8％목과단백매조화대조조。실험제1、3、5천분조행우슬관절강내주사2％、4％、8％（ w／v）목과단백매화0．3mol／LL－반광안산적혼합용액0．25 mL／kg혹등량생리염수。기록소서일반정황급우슬관절관도。소유소서수차주사1주후처사，취관절행상규HE염색급갑분알람염색，경하관찰병평분。결과조모조우슬관절출현종창、공능장애；우슬관절연골염색변천、연골층변박、기질분해파배등병이학개변，기엄중정도여목과단백매농도정정상관；대조조무상술개변。결론슬관절강내주사2％、4％、8％（ w／v）목과단백매화0．3mol／L L－반광안산적혼합용액0．25 mL／kg가건립소서불동엄중정도적슬골관절염，주기단、성공솔고。
Objective To determine the feasibility of the knee osteoarthritis ( OA) models in mice with different concentrations of papain mixed solution.Method Eighty Kunming mice, weight (45 ±3)g, were randomly divided into four groups of 20 each (2%papain group, 4%papain group, 8%papain group, and control group) and given injections into the right knees on the 1st, 3rd, 5th day respectively.The mixed solution, composed of 2%, 4%, 8%(w/v) papain and 0.3mol/L l-cysteine, and 0.9%Nacl were applied to experimental groups and control group respectively at the dose of 0.25ml/kg.All mice were sacrificed at one week after the initiation of papain injection and evaluated through recording the width of knee joints, performing the morphological observation ( toluidine bluc staining& HE staining) and histological evaluation of articular cartilage.Results Swelling and activities diminishment could be observed in the knee joints of experimental groups.The degenerative changes were demonstrated in the knee joints of all experimental groups, such as shallow staining, thinner articular cartilage and destroyed cartilage matrix, and inflammation.All these changes were much worse with increased concentration. Conclusion Different severities of OA can be established through intra-articular injections of 2%, 4%, 8% papain and 0.03mol/L l-cysteine at the dose of 0.25ml/kg.These models are of the characters of short period and a good reproducibility.
