CAJ | 학술논문

目的：定量分析 T2 mapping 技术检测早期关节软骨退变中胶原含量变化的能力，并探讨其机制和意义。方法20个家猪髌骨随机分为4组，分别经浓度100 mg/100 mL、150 mg/100 mL、200 mg/100 mL 的Ⅱ型胶原酶(酶处理组)和无酶 PBS 缓冲液(对照组)处理。处理后髌骨行 MR T2 WI 和 T2 mapping 成像。分别测定髌关节软骨浅深2层和全层的 T2值。采用 Van Gieson染色法对软骨标本进行染色，并测定各组软骨染色后的平均光密度，分析软骨 T2值与平均光密度之间的关系。结果大体病理示各组髌骨软骨与处理前相比，软骨表面均无明显变化。统计学分析表明，各处理组与对照组比较，在软骨浅层与全层 T2值以及软骨平均光密度值上均有明显差异(P <0.05)。各处理组之间相互比较，在软骨浅层 T2值上和软骨平均光密度值上任何2处理组间差异均有统计学意义；而在软骨全层 T2值两两比较上，只有100 mg/100 mL 处理组和150 mg/100 mL 处理组2组间差异无统计学意义(P >0.05)。软骨全层 T2值与软骨平均光密度的相关分析显示，两者之间呈高度负相关(r=-0.837)。结论T2 mapping技术能够很好地检测出早期骨性关节炎模型软骨中胶原成分的变化，具有良好的临床应用前景。
목적：정량분석 T2 mapping 기술검측조기관절연골퇴변중효원함량변화적능력，병탐토기궤제화의의。방법20개가저빈골수궤분위4조，분별경농도100 mg/100 mL、150 mg/100 mL、200 mg/100 mL 적Ⅱ형효원매(매처리조)화무매 PBS 완충액(대조조)처리。처리후빈골행 MR T2 WI 화 T2 mapping 성상。분별측정빈관절연골천심2층화전층적 T2치。채용 Van Gieson염색법대연골표본진행염색，병측정각조연골염색후적평균광밀도，분석연골 T2치여평균광밀도지간적관계。결과대체병리시각조빈골연골여처리전상비，연골표면균무명현변화。통계학분석표명，각처리조여대조조비교，재연골천층여전층 T2치이급연골평균광밀도치상균유명현차이(P <0.05)。각처리조지간상호비교，재연골천층 T2치상화연골평균광밀도치상임하2처리조간차이균유통계학의의；이재연골전층 T2치량량비교상，지유100 mg/100 mL 처리조화150 mg/100 mL 처리조2조간차이무통계학의의(P >0.05)。연골전층 T2치여연골평균광밀도적상관분석현시，량자지간정고도부상관(r=-0.837)。결론T2 mapping기술능구흔호지검측출조기골성관절염모형연골중효원성분적변화，구유량호적림상응용전경。
Objective To investigate the ability of T2 mapping in early detection of the change of cartilage collagen by quantitative analysis.Methods 20 porcine patellae were randomly assigned into 4 groups,3 treated groups and 1 control group.The samples of 3 treated groups were respectively immersed in PBS with 100 mg/100 mL,1 50 mg/100 mL and 200 mg/100 mL typeⅡ collagenase for 4 h,whereas control group samples in PBS for 4 h.T2 relaxation times of superficial,deep and full layers of cartilage and mean densi-ty after Van Gieson stain were measured.The correlation of T2 relaxation times and mean density were statistically analyzed.Results Gross pathology showed all articular cartilages maintained intact after treatment.T2 relaxation times of superficial and full layers and mean density increased significantly in any treated group in comparison with control group(P <0.05).Significant differences in T2 relaxation times of superficial layers and mean density were found between any two treated groups,whereas no difference of T2 re-laxation time of full layers was found between 100 mg/100 mL and 1 50 mg/100 mL treated groups(P >0.05).Pearson correlation analysis showed a close correlation(r=-0.837)between T2 relaxation times of full layers and mean density.Conclusion T2 map-ping can adequately detect the change of cartilage collagen in the early stage of osteoarthritis,and shows a good clinical application prospect.