CAJ | 학술논문

目的：考察姜黄素PLGA－TPGS纳米粒（ curcumin－loaded PLGA－TPGS nanoparticles，CPTN）体内对荷小鼠腹水型肝癌高淋巴道转移细胞HCa－F实体瘤的抑制作用。方法建立荷HCa－F细胞的小鼠实体瘤模型，尾静脉分别注射生理盐水、空白纳米粒、姜黄素溶液、5－氟尿嘧啶溶液和CPTN，7 d后取出实体瘤，测量瘤径、瘤重、测定瘤内药量并进行HE染色，全面评价CPTN对肝癌实体瘤的治疗效果。结果体内药效学实验中CPTN组和5－氟尿嘧啶溶液组的瘤体体积增长量和瘤重明显低于生理盐水（空白对照）组，差异具有显著性意义（P＜0．05）；两组抑瘤率分别为63．44％和38．14％。姜黄素溶液组和CPTN组实体瘤中的姜黄素量分别为（3．75±0．22）μg和（31．64±2．54）μg，差异具有显著性意义（P＜0．01）。 HE染色后镜下观察该组切片显示大部分细胞丢失细胞核，破碎成碎片，边缘不完整，表明其治疗肿瘤效果最佳。结论 CPTN在体内对荷HCa－F细胞的小鼠实体瘤有一定的抑制作用，且治疗效果好于5－氟尿嘧啶注射液。
목적：고찰강황소PLGA－TPGS납미립（ curcumin－loaded PLGA－TPGS nanoparticles，CPTN）체내대하소서복수형간암고림파도전이세포HCa－F실체류적억제작용。방법건립하HCa－F세포적소서실체류모형，미정맥분별주사생리염수、공백납미립、강황소용액、5－불뇨밀정용액화CPTN，7 d후취출실체류，측량류경、류중、측정류내약량병진행HE염색，전면평개CPTN대간암실체류적치료효과。결과체내약효학실험중CPTN조화5－불뇨밀정용액조적류체체적증장량화류중명현저우생리염수（공백대조）조，차이구유현저성의의（P＜0．05）；량조억류솔분별위63．44％화38．14％。강황소용액조화CPTN조실체류중적강황소량분별위（3．75±0．22）μg화（31．64±2．54）μg，차이구유현저성의의（P＜0．01）。 HE염색후경하관찰해조절편현시대부분세포주실세포핵，파쇄성쇄편，변연불완정，표명기치료종류효과최가。결론 CPTN재체내대하HCa－F세포적소서실체류유일정적억제작용，차치료효과호우5－불뇨밀정주사액。
Objective To investigate the in vivo therapeutic effects on ascitic hepatocarcinoma cell strain with high metastasis potential in lymphatic system (HCa-F) solid tumors in mice with curcumin (CM)-loaded polylactic-co-glycolic-D-α-tocopherol polyethylene glycol 1000 succinate acid nanoparticles ( CPTN ) .Methods HCa-F cells were inoculated into mice's armpit to form of liver cancer solid tumor model.Drug was intravenously injected by normal saline,empty PTN,curcu-min solutions ( CS) ,fluorouracil solutions ( FS) and CPTN respectively once a day for consecutive seven days.After the treat-ment,solid tumors were taken,measured diameter and weighed,determine CM mass in tumors,embedded in paraffin and sec-tioned.The slices were stained by HE to comprehensively evaluate the combination therapy of CPTN for the treatment of solid tumors.Results For 7 days treatment, tumor volume growth of CPTN group and FS group were significantly slower than the normal saline (blank control) group (P<0.05), CM mass of CPTN group were significantly higher than that of CS group (P<0.01).Inhibition rate of the CPTN group could reach more than 60%, and the slice stained with HE of CPTN group shows that almost all cells lost nuclei and broke into fragments with fuzzy edge.The results indicated CPTN got the best effect against liver cancer.Conclusions Results of pharmacodynamic tests in vivo showed that CPTN had a good effect for liver canc-er,and treatment of the mice in CPTN group was better than FS group.