CAJ | 학술논문

目的：研究S100A4在肺鳞癌与肺腺癌细胞系中的表达及对细胞增殖、转移、侵袭功能的影响。方法利用RT－PCR和Western Blot检测S100A4在肺鳞癌细胞系NCI－H520和肺腺癌细胞系SPC－A－1中的表达，利用siRNA干扰S100A4在两个细胞系的表达，CCK－8检测细胞增殖，划痕、Transwell实验检测细胞迁移侵袭。结果 RT－PCR与Western Blot检测结果显示S100A4在肺鳞癌及肺腺癌细胞系中均有表达，且肺鳞癌细胞系NCI－H520中S100A4表达水平低于肺腺癌细胞系SPC－A－1（P＜0．05）。细胞荧光、Western Blot 及RT－PCR验证S100A4敲除效率，CCK－8实验证明干扰S100A4后，两肺癌细胞系的增殖能力较空白对照组和阴性对照组均降低（P＜0．05）、划痕及Tranwell实验证明干扰S100A4后，两肺癌细胞系的迁移能力与对照组相比也降低（P＜0．05）。结论 S100A4在肺鳞癌细胞系与肺腺癌细胞系均有表达，且鳞癌高于腺癌，S100A4与肺癌细胞恶性进展有关，有可能成为肺癌治疗的新靶点。
목적：연구S100A4재폐린암여폐선암세포계중적표체급대세포증식、전이、침습공능적영향。방법이용RT－PCR화Western Blot검측S100A4재폐린암세포계NCI－H520화폐선암세포계SPC－A－1중적표체，이용siRNA간우S100A4재량개세포계적표체，CCK－8검측세포증식，화흔、Transwell실험검측세포천이침습。결과 RT－PCR여Western Blot검측결과현시S100A4재폐린암급폐선암세포계중균유표체，차폐린암세포계NCI－H520중S100A4표체수평저우폐선암세포계SPC－A－1（P＜0．05）。세포형광、Western Blot 급RT－PCR험증S100A4고제효솔，CCK－8실험증명간우S100A4후，량폐암세포계적증식능력교공백대조조화음성대조조균강저（P＜0．05）、화흔급Tranwell실험증명간우S100A4후，량폐암세포계적천이능력여대조조상비야강저（P＜0．05）。결론 S100A4재폐린암세포계여폐선암세포계균유표체，차린암고우선암，S100A4여폐암세포악성진전유관，유가능성위폐암치료적신파점。
Objective To explore the expression of S100A4 in lung squamous carcinoma and adenocarcinoma cells and its effection in proliferation,metastasis,invasion.Meth ods RT-PCR and Western Blot were used to detect the expression of S100A4 in lung squamous carcinoma NCI-H520 and adenocarcinoma SPC-A-1 cell lines,using siRNA to knockdown S100A4 in these two cell lines and CCK-8,wound healing assay and transwell experiment were used to detect the cell pro-liferation,migration and invasion.Results The expression of S100A4 was different in these two cell lines and the expression was lower in squamous carcinoma than adenocarcinoma (P<0.05).The transfection efficiency was detected by immuno-fluorescence,Western Blot and RT-PCR.Knocking down S100A4 significantly reduced the cell proliferation,migration which was confirmed by CCK-8,wound healing and transwell assay (P<0.05).Conclusion Expression of S100A4 in lung squamous carcinoma NCI-H520 was significantly lower than adenocarcinoma SPC-A-1 cell line.Knocking down S100A4 reduced the lung cancer cell malignant and may be a potential target for lung cancer treatment.