江西师范大学学报（自然科学版）
江西師範大學學報（自然科學版）
강서사범대학학보（자연과학판）
JOURNAL OF JIANGXI NORMAL UNIVERSITY（NATURAL SCIENCES EDITION）
2015年
2期
162-165
,共4页
超高效合相色谱-质谱法%大豆油%脂肪酸%未衍生化
超高效閤相色譜-質譜法%大豆油%脂肪痠%未衍生化
초고효합상색보-질보법%대두유%지방산%미연생화
ultra-performance convergence chromatography-mass spectrometry%soybean oil%fatty acids%underivat-ization
建立了大豆油中5种脂肪酸的超高效合相色谱-质谱（ UPC2-MS）分析方法，这5种脂肪酸分别为棕榈酸、硬脂酸、油酸、亚油酸和亚麻酸.待测物经皂化反应后，用正己烷溶解，经ACQUITY UPC2 BEH 2-EP色谱柱分离，以CO2-甲醇/乙腈（体积比为1：1）为流动相进行梯度洗脱，通过质谱检测器测定、外标法定量.方法的最低检出限为0.07 mg·L-1，脂肪酸的加标回收率为90.50%~105.38%，相对标准偏差为0.81%~2.93%.结果表明，该方法灵敏度高、分离效果好、测定结果准确.
建立瞭大豆油中5種脂肪痠的超高效閤相色譜-質譜（ UPC2-MS）分析方法，這5種脂肪痠分彆為棕櫚痠、硬脂痠、油痠、亞油痠和亞痳痠.待測物經皂化反應後，用正己烷溶解，經ACQUITY UPC2 BEH 2-EP色譜柱分離，以CO2-甲醇/乙腈（體積比為1：1）為流動相進行梯度洗脫，通過質譜檢測器測定、外標法定量.方法的最低檢齣限為0.07 mg·L-1，脂肪痠的加標迴收率為90.50%~105.38%，相對標準偏差為0.81%~2.93%.結果錶明，該方法靈敏度高、分離效果好、測定結果準確.
건립료대두유중5충지방산적초고효합상색보-질보（ UPC2-MS）분석방법，저5충지방산분별위종려산、경지산、유산、아유산화아마산.대측물경조화반응후，용정기완용해，경ACQUITY UPC2 BEH 2-EP색보주분리，이CO2-갑순/을정（체적비위1：1）위류동상진행제도세탈，통과질보검측기측정、외표법정량.방법적최저검출한위0.07 mg·L-1，지방산적가표회수솔위90.50%~105.38%，상대표준편차위0.81%~2.93%.결과표명，해방법령민도고、분리효과호、측정결과준학.
The 5 fatty acids in soybean oil were analyzed using ultra-performance convergence chromatography,in-cluding palmitic acid,stearic acid,oleic acid,linoleic acid and linolenic acid. The sample was dissolved by n-hexane after saponification reaction. The fatty acids were separated on the column of ACQUITY UPC2 BEH 2-EP by gradient elution with carbon dioxide and V( methano): V( acetonitrile)=1: 1 system,and finally detected by MS detector. The limits of detection of target compounds in the method were 0. 07 mg·L-1 . The recoveries were from 90. 50%to 105. 38% at three spiked levels with the relative standard deviations lower than 3. 0%. The method,with high sensitivity,good separation effect and high recovery,was successfully used to detect the underivatization fatty acids in soybean oil.
