中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
12期
1805-1811
,共7页
胡涛%徐铭恩%严明%郭淼%石然%周青青
鬍濤%徐銘恩%嚴明%郭淼%石然%週青青
호도%서명은%엄명%곽묘%석연%주청청
生物材料%骨生物材料%聚乳酸羟基乙酸%珍珠粉%磷酸三钙%低温层积制造%国家自然科学基金
生物材料%骨生物材料%聚乳痠羥基乙痠%珍珠粉%燐痠三鈣%低溫層積製造%國傢自然科學基金
생물재료%골생물재료%취유산간기을산%진주분%린산삼개%저온층적제조%국가자연과학기금
Calcium Phosphates%Tissue Engineering%Biocompatible Materials
背景:研究证实,在聚乳酸羟基乙酸中加入磷酸三钙或珍珠粉可以使两者的性能互补,不仅为细胞提供良好的生长环境,而且能使细胞更快、更好地生长。目的:通过低温层积制造技术制备以聚乳酸羟基乙酸为基体、复合珍珠粉或磷酸三钙的支架材料。方法:采用低温层积制造技术制备聚乳酸羟基乙酸与珍珠粉或磷酸三钙质量比分别为10∶0、5∶2、7∶3和6∶4的聚乳酸羟基乙酸/珍珠粉或聚乳酸羟基乙酸/磷酸三钙复合支架,检测支架的微观结构、接触角、压缩弹性模量。将生长至基本融合的MC3T3-E1细胞以1×104/cm3结果与结论:纯聚乳酸羟基乙酸支架具有相互交联贯通的微孔结构,微孔大小3-15μm;5∶2聚乳酸羟基乙酸/珍珠粉或5∶2聚乳酸羟基乙酸/磷酸三钙支架具有较好的连续微孔结构,孔径大小为10-25μm。随着珍珠粉或磷酸三钙含量的增加,复合支架的亲水性增加。加入珍珠粉或磷酸三钙能够提高复合支架的压缩力学性能,但随着含量增加,支架力学性能呈现先增强后减弱。珍珠粉或磷酸三钙的加入,改善了聚乳酸羟基乙酸的细胞亲和力,改善了支架的生物相容性,其中以5∶2聚乳酸羟基乙酸/珍珠粉复合支架生物相容性最好。的密度接种至纯聚乳酸羟基乙酸无孔支架、纯聚乳酸羟基乙酸有孔支架、5∶2聚乳酸羟基乙酸/珍珠粉和5∶2聚乳酸羟基乙酸/磷酸三钙支架上,接种1,3 h,采用流式细胞仪检测细胞黏附率;接种1,4,7 d,利用Alamar Blue法检测细胞增殖。
揹景:研究證實,在聚乳痠羥基乙痠中加入燐痠三鈣或珍珠粉可以使兩者的性能互補,不僅為細胞提供良好的生長環境,而且能使細胞更快、更好地生長。目的:通過低溫層積製造技術製備以聚乳痠羥基乙痠為基體、複閤珍珠粉或燐痠三鈣的支架材料。方法:採用低溫層積製造技術製備聚乳痠羥基乙痠與珍珠粉或燐痠三鈣質量比分彆為10∶0、5∶2、7∶3和6∶4的聚乳痠羥基乙痠/珍珠粉或聚乳痠羥基乙痠/燐痠三鈣複閤支架,檢測支架的微觀結構、接觸角、壓縮彈性模量。將生長至基本融閤的MC3T3-E1細胞以1×104/cm3結果與結論:純聚乳痠羥基乙痠支架具有相互交聯貫通的微孔結構,微孔大小3-15μm;5∶2聚乳痠羥基乙痠/珍珠粉或5∶2聚乳痠羥基乙痠/燐痠三鈣支架具有較好的連續微孔結構,孔徑大小為10-25μm。隨著珍珠粉或燐痠三鈣含量的增加,複閤支架的親水性增加。加入珍珠粉或燐痠三鈣能夠提高複閤支架的壓縮力學性能,但隨著含量增加,支架力學性能呈現先增彊後減弱。珍珠粉或燐痠三鈣的加入,改善瞭聚乳痠羥基乙痠的細胞親和力,改善瞭支架的生物相容性,其中以5∶2聚乳痠羥基乙痠/珍珠粉複閤支架生物相容性最好。的密度接種至純聚乳痠羥基乙痠無孔支架、純聚乳痠羥基乙痠有孔支架、5∶2聚乳痠羥基乙痠/珍珠粉和5∶2聚乳痠羥基乙痠/燐痠三鈣支架上,接種1,3 h,採用流式細胞儀檢測細胞黏附率;接種1,4,7 d,利用Alamar Blue法檢測細胞增殖。
배경:연구증실,재취유산간기을산중가입린산삼개혹진주분가이사량자적성능호보,불부위세포제공량호적생장배경,이차능사세포경쾌、경호지생장。목적:통과저온층적제조기술제비이취유산간기을산위기체、복합진주분혹린산삼개적지가재료。방법:채용저온층적제조기술제비취유산간기을산여진주분혹린산삼개질량비분별위10∶0、5∶2、7∶3화6∶4적취유산간기을산/진주분혹취유산간기을산/린산삼개복합지가,검측지가적미관결구、접촉각、압축탄성모량。장생장지기본융합적MC3T3-E1세포이1×104/cm3결과여결론:순취유산간기을산지가구유상호교련관통적미공결구,미공대소3-15μm;5∶2취유산간기을산/진주분혹5∶2취유산간기을산/린산삼개지가구유교호적련속미공결구,공경대소위10-25μm。수착진주분혹린산삼개함량적증가,복합지가적친수성증가。가입진주분혹린산삼개능구제고복합지가적압축역학성능,단수착함량증가,지가역학성능정현선증강후감약。진주분혹린산삼개적가입,개선료취유산간기을산적세포친화력,개선료지가적생물상용성,기중이5∶2취유산간기을산/진주분복합지가생물상용성최호。적밀도접충지순취유산간기을산무공지가、순취유산간기을산유공지가、5∶2취유산간기을산/진주분화5∶2취유산간기을산/린산삼개지가상,접충1,3 h,채용류식세포의검측세포점부솔;접충1,4,7 d,이용Alamar Blue법검측세포증식。
BACKGROUND:The study confirmed that adding tricalcium phosphate or pearl powder in polylactic-co-glycolic acid can complement the performance of both, which provides a good environment for cels and makes a faster and better growth of cels. OBJECTIVE:We used polylactic-co-glycolic acid as matrix, composited with pearl powder or tricalcium phosphate to prepare scaffolds by low-temperature deposition manufacturing. METHODS:Low-temperature deposition manufacturing was utilized to prepare composite scaffold of polylactic-co-glycolic acid/pearl powder or polylactic-co-glycolic acid/tricalcium phosphate at the ratio of 10:0, 5:2, 7:3 and 6:4. Microstructure, contact angle and compression modulus of elasticity of scaffolds were detected. MC3T3-E1 cels basicaly fused at 1×104/cm3 were seeded in the pure nonporous polylactic-co-glycolic acid scaffold, pure polylactic-co-glycolic acid scaffold with holes, polylactic-co-glycolic acid/pearl powder at 5:2 and polylactic-co-glycolic acid/tricalcium phosphate at 5:2 separately for 1 and 3 hours. Cel adhesion rate was detected using flow cytometry. After incubation for 1, 4 and 7 days, cel proliferation was measured using Alamar Blue method. RESULTS AND CONCLUSION:Pure polylactic-co-glycolic acid scaffold had cross-linked microporous structure, with pore size of 3-15 μm. Scaffolds ofpolylactic-co-glycolic acid/pearl powder at 5:2 or polylactic-co-glycolic acid/tricalcium phosphate at 5:2 had good continuous porous structure, with pore size of 10-25 μm. With increased content of pearl powder or tricalcium phosphate, the hydrophilicity of the composite scaffold increased. The addition of pearl powder or tricalcium phosphate could elevate compressive mechanical properties of the composite scaffold. With increased content, the mechanical property of the scaffold enhanced and then reduced. The addition of pearl powder or tricalcium phosphate improved the celular affinity of polylactic-co-glycolic acid and the biocompatibility of the scaffold. The biocompatibility of polylactic-co-glycolic acid/pearl powder scaffold at 5:2 was the best.