CAJ | 학술논문

背景：合肥大学材料系和中国科学院安徽光学密精机械研究所联合研究应用脉冲激光沉积合成技术制备出一种新型的纳米羟基磷灰石人工心脏机械瓣膜。目的：观察纳米羟基磷灰石人工心脏机械瓣膜与人脐静脉血管内皮细胞的相容性。方法：将体外分离培养的传2-4代人脐静脉血管内皮细胞悬液接种于纳米羟基磷灰石人工心脏机械瓣膜上，培养3，7，21 d后，扫描电子显微镜下观察细胞在纳米羟基磷灰石人工心脏机械瓣膜上的生长情况。分别采用纳米羟基磷灰石人工心脏机械瓣膜常温浸提液、纳米羟基磷灰石人工心脏机械瓣膜高温浸提液、高密度聚乙烯浸提液及苯酚溶液培养人脐静脉血管内皮细胞，72 h后采用MTT法检测细胞增殖情况。结果与结论：扫描电镜观察培养3 d时，人脐静脉血管内皮细胞呈梭形或多边形，伸出突起黏附于纳米羟基磷灰石人工心脏机械瓣膜上；7 d时，可见瓣膜表面细胞伸展充分，连接融合；21 d时，细胞成片融合，牢固覆盖于瓣膜表面，部分区域形成细胞外基质。MTT检测结果显示，纳米羟基磷灰石人工心脏机械瓣膜对人脐静脉血管内皮细胞无细胞毒性，具有良好的细胞相容性。
배경：합비대학재료계화중국과학원안휘광학밀정궤계연구소연합연구응용맥충격광침적합성기술제비출일충신형적납미간기린회석인공심장궤계판막。목적：관찰납미간기린회석인공심장궤계판막여인제정맥혈관내피세포적상용성。방법：장체외분리배양적전2-4대인제정맥혈관내피세포현액접충우납미간기린회석인공심장궤계판막상，배양3，7，21 d후，소묘전자현미경하관찰세포재납미간기린회석인공심장궤계판막상적생장정황。분별채용납미간기린회석인공심장궤계판막상온침제액、납미간기린회석인공심장궤계판막고온침제액、고밀도취을희침제액급분분용액배양인제정맥혈관내피세포，72 h후채용MTT법검측세포증식정황。결과여결론：소묘전경관찰배양3 d시，인제정맥혈관내피세포정사형혹다변형，신출돌기점부우납미간기린회석인공심장궤계판막상；7 d시，가견판막표면세포신전충분，련접융합；21 d시，세포성편융합，뢰고복개우판막표면，부분구역형성세포외기질。MTT검측결과현시，납미간기린회석인공심장궤계판막대인제정맥혈관내피세포무세포독성，구유량호적세포상용성。
BACKGROUND:A new type of nano-hydroxyapatite artificial mechanical heart valve has been developed using pulsed laser deposition technology at the Department of Materials, Hefei University and Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, China. OBJECTIVE:To investigate the compatibility of nano-hydroxyapatite artificial mechanical heart valve with human umbilical vein endothelial cels. METHODS:Human umbilical vein endothelial cels were in vitroisolated, cultured and passaged to the 2-4 generations, and then the cel suspension was inoculated onto the nano-hydroxyapatite artificial mechanical heart valve. After 3, 7, 12 days of culture, the cel growth on the artificial mechanical heart valve was observed under scanning electron microscope. In addition, the human umbilical vein endothelial cels were respectively cultured in room-temperature and high-temperature extract liquids of nano-hydroxyapatite artificial mechanical heart valve, high-density polyethylene and phenol solution extracts for 72 hours, and then, the proliferation of cels was detected by MTT method. RESULTS AND CONCLUSION:Under the scanning electron microscope, the human umbilical vein endothelial cels were fusiform- or polygon-shaped with protuberances adhered to the artificial mechanical heart value at 3 days of culture; the cels were stretched thoroughly and fused at 7 days of culture; and the cels were confluent to pieces that tightly overlaid the heart valve surface and the extracelular matrix was formed localy at 21 days of culture. Results from MTT test displayed that the nano-hydroxyapatite artificial mechanical heart valve had no cytotoxicity to the human umbilical vein endothelial cels, indicating a good cytocompatibility.