CAJ | 학술논문

背景：与有机荧光染料和荧光蛋白相比，稀土纳米材料具有更高的灵敏性和更好的光学稳定性，并且具有更低的细胞毒性。目的：探讨(Y 0.95 Eu 0.05)2 O 3方法：采用溶胶-凝胶法合成(Y稀土纳米材料的体外细胞相容性，以及氨基化修饰对材料细胞相容性的影响。0.95 Eu 0.05)2 O 3稀土纳米材料，并进行氨基化修饰。将10，25，50，100，200 mg/L的氨基化与非氨基化(Y0.95Eu0.05)2O3稀土纳米材料悬液分别与SD大鼠血管平滑肌细胞共培养3 d， CCK-8法检测细胞增殖；将100 mg/L的氨基化与非氨基化(Y0.95Eu0.05)2O3结果与结论：随着材料悬液质量浓度的升高，血管平滑肌细胞存活率呈逐渐减少趋势；在材料质量浓度为10，25，50 mg/L时，氨基化组细胞存活率明显高于未氨基化组(P<0.05)；当材料质量浓度高达200 mg/L时，未氨基化组细胞存活率降到76%，氨基化组细胞存活率仍然在80%以上，两组比较差异有显著性意义(P<0.05)。氨基化组L929细胞生长状况良好，凋亡细胞数量较少；未氨基化组生长较稀疏，细胞凋亡数量较多，显示出轻微的细胞毒性。表明氨基化修饰可以有效提高(Y稀土纳米材料悬液分别与L929细胞共培养48 h，荧光染色观察细胞凋亡。0.95Eu0.05)2O3稀土纳米材料的细胞相容性。
배경：여유궤형광염료화형광단백상비，희토납미재료구유경고적령민성화경호적광학은정성，병차구유경저적세포독성。목적：탐토(Y 0.95 Eu 0.05)2 O 3방법：채용용효-응효법합성(Y희토납미재료적체외세포상용성，이급안기화수식대재료세포상용성적영향。0.95 Eu 0.05)2 O 3희토납미재료，병진행안기화수식。장10，25，50，100，200 mg/L적안기화여비안기화(Y0.95Eu0.05)2O3희토납미재료현액분별여SD대서혈관평활기세포공배양3 d， CCK-8법검측세포증식；장100 mg/L적안기화여비안기화(Y0.95Eu0.05)2O3결과여결론：수착재료현액질량농도적승고，혈관평활기세포존활솔정축점감소추세；재재료질량농도위10，25，50 mg/L시，안기화조세포존활솔명현고우미안기화조(P<0.05)；당재료질량농도고체200 mg/L시，미안기화조세포존활솔강도76%，안기화조세포존활솔잉연재80%이상，량조비교차이유현저성의의(P<0.05)。안기화조L929세포생장상황량호，조망세포수량교소；미안기화조생장교희소，세포조망수량교다，현시출경미적세포독성。표명안기화수식가이유효제고(Y희토납미재료현액분별여L929세포공배양48 h，형광염색관찰세포조망。0.95Eu0.05)2O3희토납미재료적세포상용성。
BACKGROUND:Compared with organic fluorescent dyes and fluorescent proteins, rare earth nanomaterials have higher sensitivity, better optical stability, and lower cytotoxicity. OBJECTIVE: To investigate thein vitro cytocompatibility of (Y0.95Eu0.05)2O3 METHODS:(Y rare earth nanomaterial and the effect of amine functionalization on the material cytocompatibility. 0.95Eu0.05)2O3 nanomaterials were synthesized using sol-gel method, and underwent amine functionalization. Amine-functionalized samples (10, 25, 50, 100, 200 mg/L) and non-amine-functionalized (Y0.95Eu0.05)2O3 rare earth nanomaterial suspensions were co-cultured with vascular smooth muscle cels of Sprague-Dawley rats for 3 days. Cel proliferation was detected using Cel Counting Kit-8 assay. 100 mg/L amine-functionalized samples and non-amine-functionalized (Y0.95Eu0.05)2O3 rare earth nanomaterial suspensions were co-cultured with L929 cels for 48 hours. Cel apoptosis was observed using fluorescence staining. RESULTS AND CONCLUSION:With increased mass concentration of material suspension, survival rate of vascular smooth muscle cels was gradualy reduced. At the mass concentration of 10, 25, 50 mg/L, cel survival rate was significantly higher in the amine-functionalized group than in the non-amine-functionalized group (P < 0.05). When the mass concentration of materials reached 200 mg/L, the cel survival rate decreased to 76% in the non-amine-functionalized group, but it was stil above 80% in the amine-functionalized group, showing significant differences between the two groups (P < 0.05). The growth of L929 cels was good and the number of apoptotic cels was less in the amine-functionalized group. Cels were sparse and the number of cel apoptosis was more in the non-amine-functionalized group, showing slight cytotoxicity. These data verified that amine-functionalization can improve cytocompatibility of (Y0.95Eu0.05)2O3 rare earth nanomaterial.