CAJ | 학술논문

采用 HPLC‐ESI‐MS和傅里叶红外光谱法对五种不同产地中药肉苁蓉进行了分析，采用 HPLC‐ESI‐M S检测了中药肉苁蓉中荒漠肉苁蓉苷A、松果菊苷、毛蕊花糖苷、异毛蕊花糖苷、2’‐乙酰基毛蕊花糖苷、荒漠肉苁蓉苷C、管花苷B七种有效成分的含量。采用傅里叶红外光谱法，以五种药用植物样品的红外指纹图谱为依据，计算出所测样品的共有峰率和变异峰率。建立了五种药用植物的共有峰率和变异峰率双指标序列。结果显示两种方法对药材评价结果基本一致。采用傅里叶变换红外光谱法亦可区分肉苁蓉的常见伪品锁阳。HPLC‐ESI‐MS具有高通量、高灵敏度、分析快速等优点，可以通过主要化学成分含量的测定对肉苁蓉进行质量评价。傅里叶红外光谱法可以对样品进行无损处理，不需要对药材进行复杂的提取分离过程即可获得肉苁蓉红外光谱特征，其光谱的吸收峰强度与峰形是各种官能团互相作用的结果，是肉苁蓉多组分红外光谱的叠加。我们能从整体水平分析谱图特征峰，具有分析速度快，重现性好，非破坏性和样品量小，制样简单，专属性强，节约成本，保护环境等优点，符合中药向综合分析和整体分析的发展趋势。HPLC‐ESI‐MS和傅里叶红外光谱法联合应用，两种方法互为补充，为鉴别中药的真伪，探讨中药质量评价提供了一种新方法。
채용 HPLC‐ESI‐MS화부리협홍외광보법대오충불동산지중약육종용진행료분석，채용 HPLC‐ESI‐M S검측료중약육종용중황막육종용감A、송과국감、모예화당감、이모예화당감、2’‐을선기모예화당감、황막육종용감C、관화감B칠충유효성분적함량。채용부리협홍외광보법，이오충약용식물양품적홍외지문도보위의거，계산출소측양품적공유봉솔화변이봉솔。건립료오충약용식물적공유봉솔화변이봉솔쌍지표서렬。결과현시량충방법대약재평개결과기본일치。채용부리협변환홍외광보법역가구분육종용적상견위품쇄양。HPLC‐ESI‐MS구유고통량、고령민도、분석쾌속등우점，가이통과주요화학성분함량적측정대육종용진행질량평개。부리협홍외광보법가이대양품진행무손처리，불수요대약재진행복잡적제취분리과정즉가획득육종용홍외광보특정，기광보적흡수봉강도여봉형시각충관능단호상작용적결과，시육종용다조분홍외광보적첩가。아문능종정체수평분석보도특정봉，구유분석속도쾌，중현성호，비파배성화양품량소，제양간단，전속성강，절약성본，보호배경등우점，부합중약향종합분석화정체분석적발전추세。HPLC‐ESI‐MS화부리협홍외광보법연합응용，량충방법호위보충，위감별중약적진위，탐토중약질량평개제공료일충신방법。
Five samples of Cistanches Herba from different places were analyzed by HPLC‐ESI‐MS and FTIR methods .The effective compositions in Cistanches Herba including cistanoside A ,echinacoside ,acteoside ,isoacteoside ,2’‐actylacteoside , cistanoside C and tubluoside B were determined by HPLC‐MS .The common peak ratio and variant peak ratio were calculated by FTIR spectroscopy of the five samples and the dual index sequence of common peak ratio and variant peak ratio were established . The results showed that the evaluation results of the samples by the two methods were the same . The general fake plant Cynomorii Herba could be identified by FTIR .HPLC‐ESI‐MS ,which has high sensitivity and rapid determination procedure , can be used to evaluate quality of Cistanches Herba by quantitative analysis of the primary compositions .FTIR is a non‐destruc‐tive analysis method without complicated extraction and separation procedures to the samples .The absorption strength and the absorption shape were the synergistic effect of the functional groups and the nestification of the components in Cistanches Herba . The provided method has some advantages such as rapid analysis process ,good reproducibility ,non‐destructive ,small quantity of sample ,simple treatment ,good specificity ,low‐cost and environment‐friendly .The method meets the trend of complex analy‐sis and whole analysis for the Chinese medicines .Combination of FTIR and HPLC‐ESI‐MS was a good method for identification and evaluation of quality of Chinese medicines .